N-containing saccharides and method for the synthesis of N-containing saccharides from amino-deoxy-disaccharides and amino-deoxy-oligosaccharides
AssigneeBioflexin AB
InventorKurt G. I. Nilsson
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Abstract
Synthesis of an amino-disaccharide, amino-oligosaccharide or a derivative thereof, characterized in that a monosaccharide, a disaccharide, an oligosaccharide, a glycoside or a derivative thereof, in the presence of a glycosidase as catalyst, is reacted with an amino-deoxy-saccharide or a derivative thereof, and that the amino-saccharide is isolated from the product mixture directly or after chemical/enzymatic modification.
Claims27
A method of producing an amino-deoxy di- or oligosaccharide compound which either consists of or is a fragment or analog of a carbohydrate part in a glycoconjugate, said method comprising
- (1) reacting
- (a) at least one donor substance comprising a glycoside in which an aglycon of said glycoside is glycosidically bound fluorine or is an O--, N--, C-- or S-glycosidically bound aliphatic or aromatic compound,
- (b) at least ore acceptor substance comprising an amino-deoxy mono-, di- , or oligosaccharide, or glycoside thereof, and
- (c) an E.C. group 3.2 glycosidase to form an amino-deoxy di- or oligosaccharide compound,
- (d) optionally isolating said amino-deoxy di- or oligosaccharide compound.
A method of producing an amino-deoxy di- or oligosaccharide compound which either consists of or is a fragment or analog of the carbohydrate part in a glycoconjugate, said method comprising
- (1) reacting
- (a) at least one mono-, di- or oligosaccharide, glycoside, or derivative thereof as donor substance,
- (b) at least one acceptor substance comprising an amino-deoxy mono-, di-, oligosaccharide, glycoside or derivative thereof, and
- (c) an E.C. group 3.2 glycosidase to form said amino-deoxy di- or oligosaccharide compound, and
- (2) optionally isolating said amino-deoxy di- or oligosaccharide compound.
A method of synthesizing an amino-disaccharide, amino-oligosaccharide or a derivative thereof, comprising reacting a monosaccharide, disaccharide, oligosaccharide, glycoside or derivative thereof in the presence of a glycosidase as catalyst with an amino-deoxy-saccharide or derivative thereof and optionally isolating the amino-saccharide from the product mixture directly or after further chemical/enzymatic modification.
Dependencies are inferred from the claim text.