Growth arrest homebox gene
AssigneeCase Western Reserve University
InventorsDavid H. Gorski, Kenneth Walsh
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Abstract
A novel growth arrest homeobox gene has been discovered and the nucleotide sequences have been determined in both the rat and the human. The expression of the novel homeobox gene inhibits vascular smooth muscle cell growth. The growth arrest homeobox gene hereinafter referred to as the "Gax gene" and its corresponding proteins are useful in the study of vascular smooth muscle cell proliferation and in the treatment of blood vessel diseases that result from excessive smooth muscle cell proliferation, particularly after balloon angioplasty.
Claims27
An isolated DNA molecule comprising a nucleotide sequence that encodes a mammalian Gax protein, said nucleotide sequence selected from the group consisting of a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:2 and a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:4.
An isolated DNA molecule comprising a nucleotide sequence encoding a human Gax protein that inhibits proliferation of vascular smooth muscle cells, said nucleotide sequence being 941 nucleotides in length, wherein said nucleotide sequence starting at the 3' end comprises the following fragments:
- (a) a fragment of clone 6 comprising nucleotides 699 to 941 of said nucleotide sequence;
- (b) a fragment of clone 23 comprising nucleotides 231 to 698 of said nucleotide sequence;
- (c) a fragment of clone 117 comprising nucleotides 119 to 230 of said nucleotide sequence;
- (d) a fragment of clone 131 comprising nucleotides 1 to 118 of said nucleotide sequence.
Dependencies are inferred from the claim text.