Immunologic detection of factor VA fragments in hemorrhagic and thrombotic clinical settings
AssigneeUniversity of Vermont
InventorsKenneth G. Mann, Michael Kalafatis
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Abstract
The present invention is directed to immunochemical detection procedures, e.g., using both Western blotting and direct immunoassays, for fragments of Factor Va, which can thus be used; (a) in a predictive manner to evaluate the existence and/or extent of a thrombotic complication; (b) to monitor the efficacy of prophylaxis for a thrombotic condition; and (c) as a means to evaluate potential risk of hemorrhage during thrombolytic therapy.
Claims3
A method of detecting factor Va related thrombotic or hemorrhagic events in a mammal, the method comprising:
- a) obtaining blood or plasma from the mammal;
- and
- b) monitoring by an immunoassay the mammalian blood or plasma for one or more fragments of factor Va selected from the group consisting of the cleavage fragments of the heavy chain at Arg.sup.306 and Arg.sup.506 wherein the cleavage fragments include fragments having residues 1-306 and 307-506 of factor Va,
- wherein the detection of the one or more fragments is indicative of the thrombotic or hemorrhagic events in the mammal.
A method of detecting factor Va related thrombotic or hemorrhagic events in a mammal, the method comprising:
- a) obtaining blood or plasma from the mammal;
- and
- b) monitoring by an immunoassay the mammalian blood or plasma for one or more fragments of factor Va selected from cleavage fragments of the heavy chain at Arg.sup.306,
- wherein the detection of the one or more fragments is indicative of the thrombotic or hemorrhagic events in the mammal.
Dependencies are inferred from the claim text.