Method of detection of neoplastic cells
AssigneeThe Johns Hopkins University School of Medicine
InventorsDavid Sidransky, Stephen B. Baylin
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Abstract
Methylation of p16 DNA and a resultant decrease in p16 gene expression is associated with transcriptional block and is associated with a variety of neoplasms. A method for detecting a neoplasm in a subject by detecting methylation of 5'CpG islands in p16 DNA, or detecting p16 mRNA or polypeptide levels in a sample is also provided.
Claims15
A method for detecting the presence or absence of human chromosome 9p21 or fragments thereof comprising contacting a sample containing human chromosomal DNA with a polynucleotide of FIG. 1a (SEQ ID NO:1), and detecting the hybridization of the chromosomal DNA with the polynucleotide of FIG. 1a (SEQ ID NO:
- 1).
A method for detecting a cell proliferative disorder in a tissue of a subject comprising contacting a target cellular component of the tissue with a reagent which detects a 5'ALT mediated alternate p16 transcript or truncated p16 polypeptide and detecting said alternate transcript or truncated polypeptide, wherein the alteration is indicative of a cell proliferative disorder.
Dependencies are inferred from the claim text.