Matrix substrate for a viable body tissue-derived prosthesis and method for making the same
AssigneeSt. Jude Medical, Inc.
InventorsNanette H. Bishopric, Linda Dousman, Yung-mae M. Yao
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Abstract
Disclosed is a process for treating body tissues such as vascular structures to remove barriers to recellularization before and after implantation in a human host. The body tissue, for example a heart valve, is subjected to a hypotonic/hypertonic treatment, then treated with enzymes to provide a collagen- and elastin-containing matrix suitable for recellularization.
Claims10
A method of decellularizing a body tissue to form an extracellular matrix having substantially intact collagen and elastin components, said matrix being adapted for recellularization in vitro or in vivo, comprising subjecting said body tissue to incubation in a hypotonic solution followed by incubation in a hypertonic solution or incubation in a hypertonic solution followed by incubation in a hypotonic solution, then treating said body tissue with an enzyme-based solution comprising lipase and deoxyribonuclease.
Dependencies are inferred from the claim text.