Borna disease virus-specific DNA and proteins
AssigneeThe Johns Hopkins University
InventorsJanice Clements, Opendra Narayan, Susan Vandewoude, Juergen Richt
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Abstract
Borna disease virus (BDV) causes a rare neurological disease in horses and sheep. A subtractive cDNA expression library was constructed with poly A-selected RNA from a BDV Infected MDCK cell line. A clone (B8) was isolated that specifically hybridizes to RNA isolated from BDV-infected brain tissue and BDV-infected cell lines. This clone hybridizes to four BDV-specific positive strand RNAs and one negative strand RNA in BDV-infected rat brain. Nucleotide sequence analysis of the clone suggests that it represents a full length mRNA which contains several open reading frames. The Borna Disease Virus DNA sequences as well as proteins encoded by the BDV DNA sequences are provided.
Claims9
A DNA molecule consisting of the nucleic acid sequence of SEQ ID NO:1.
An isolated DNA molecule that hybridizes to the nucleotide sequence of SEQ ID NO:1 under stringent conditions.
Dependencies are inferred from the claim text.