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US 5,854,404 AGranted
Antitumor substance extracted from grifola
AssigneeYukiguni Maitake Co., Ltd.
InventorsHiroaki Nanba, Keiko Kubo
Life of this patent
Estimating the term…
Abstract
An antitumor substance having high immunopotentiating activity, extracted and fractionated from mycelia or fruit bodies of Grifola with water can be obtained by adding alcohol to its extract at a final concentration of 20 to 60%, preferably at a final concentration of 20 to 50% by volume (low-concentration addition) to remove floating or adhering matter from it.
Claims17
A glucan/protein complex produced by the steps of:
- (a) thermally extracting mycelia or fruit bodies of Grifola with water at a temperature of from 50.degree. C. to 135.degree. C.;
- (b) adding alcohol to the resulting water-soluble extract at a final concentration of 20 to 60% by volume, allowing said extract to stand in a vessel at a temperature of 1.degree. to 25.degree. C., and removing floating matter on the liquid or in the liquid or matter adhered to the vessel wall;
- and
- (c) addition alcohol to said extract at a final concentration of 80 to 99% by volume, allowing said extract to stand at 1.degree. to 25.degree. C., and recovering said glucan/protein complex in the form of precipitates.
1 more element
A glucan/protein complex produced by the steps of:
- (a) thermally extracting mycelia or fruit bodies of Grifola with water at a temperature of from 50.degree. C. to 135.degree. C.;
- (b) adding alcohol to the resulting water-soluble extract at a final concentration of 20 to 50% by volume, allowing said extract to stand in a vessel at a temperature of 1.degree. to 5.degree. C., and removing floating matter on the liquid or in the liquid or matter adhered to the vessel wall;
- and
- (c) adding alcohol to said extract at a final concentration of 80 to 90% by volume, allowing said extract to stand at 1.degree. to 5.degree. C., and recovering said glucan/protein complex in the form of precipitates.
1 more element
A glucan/protein complex produced by the steps of:
- (a) thermally extracting mycelia or fruit bodies of Grifola with water at a temperature of from 50.degree. C. to 135.degree. C.;
- (b) adding alcohol to the resulting water-soluble extract at a final concentration of 20 to 60% by volume, allowing said extract to stand in a vessel at a temperature of 1.degree. to 25.degree. C., and removing floating matter on the liquid or in the liquid or matter adhered to the vessel wall;
- and
- (c) concentrating said extract to form said glucan/protein complex in the form of precipitates, or concentrating said extract into dryness to form said glucan/protein complex.
1 more element
A glucan/protein complex produced by the steps of:
- (a) thermally extracting mycelia or fruit bodies of Grifola with water at a temperature of from 50.degree. C. to 135.degree. C.;
- (b) adding alcohol to the resulting water-soluble extract at a final concentration of 20 to 50% by volume, allowing said extract to stand in a vessel at a temperature of 1.degree. to 5.degree. C., and removing floating matter on the liquid or in the liquid or matter adhered to the vessel wall;
- and
- (c) concentrating the solution to form said glucan/protein complex in the form of precipitates, or concentrating said extract into dryness to form said glucan/protein complex.
1 more element
Dependencies are inferred from the claim text.