US 5,854,403 A · Method for isolation of highly pure von Willebrand Factor

Abstract

The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant rvWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which comprises mulitimers with a high structural integrity.

Claims16

Dependent claims

A method for purifying recombinant von Willebrand Factor, comprising:

(a) contacting the recombinant von Willebrand Factor with a quaternary amino type anion exchanger, wherein the contacting is undertaken using buffer having a salt concentration less than 270 mM in the absence of stabilizers and free amino acid residues;
and
(b) eluting the recombinant von Willebrand Factor by increasing the salt concentration above 270 mM, wherein the von Willebrand Factor is in the form of multimers having high structural integrity.

Dependencies are inferred from the claim text.

Method for isolation of highly pure von Willebrand Factor

Life of this patent

Method for isolation of highly pure von Willebrand Factor

Life of this patent

Abstract

Claims16