Loading patent…
AssigneeLEK, tovarna farmacevtskih in kemicnih izdelkov, d.d.
InventorsRok Grahek, Andrej Bastarda
Estimating the term…
The present invention discloses a new method for the purification of vancomycin hydrochloride by preparative HPLC (method of displacement chromatography), whereby the chromatographic purity of the product is essentialy improved. The chromatography is performed on a reverse stationary phase with a mobile phase consisting of an organic or inorganic acid or of a buffer with possible additives, with different displacing agents, at a defined pH and temperature as well as the amount and concentration of vancomycin hydrochloride. The process is distinguished by the excellent yield and exceptional chromatographic purity 95.5% area of the obtained product and, besides, it represents an ecologically irreproachable process. The vancomycin hydrochloride purified according to the present invention is useful for all types of application since the portion of impurities it contains is for one third lower than in hitherto known commercially available products.
A method for the purification of vancomycin antibiotics by displacement chromatography characterized in that the stationary phase is a natural or synthetic reverse phase, the mobile phase is a diluted acid or buffer and the displacing agent is an alcohol an oxyalcohol (alcohol-ether), an alkali metal alkyl sulfate or derivative thereof or a tetraalkylammonium halide.
Dependencies are inferred from the claim text.