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US 5,854,389 AGranted
Process for preparing modified proteins
AssigneeThe Scripps Research Institute
InventorsStephen B. H. Kent, Martina Schnolzer-Rackwitz
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Abstract
Process for the preparation of modified proteins comprising the coupling of a first peptide segment having a haloacyl group at the N-terminus thereof with a second peptide segment having a carbonylthiol group at the C-terminus thereof are disclosed. Novel modified proteins produced by the process are also disclosed.
Claims19
A process for preparing a modified protein comprising the steps of:
- a. sequentially coupling amino acids or amino acid analogs to a terminal amino acid or amino acid analog bound to a first resin support to form a first peptide segment-resin, the first peptide segment comprising from about two to about one hundred amino acid residues;
- b. covalently attaching a haloacyl moiety to the N-terminus of the peptide segment-resin to form a haloacylpeptide segment bound to the first resin support;
- c. cleaving the haloacylpeptide segment from the first resin support;
- d. sequentially coupling amino acids or amino acid analogs to a terminal amino acid or amino acid analog bound to a second resin support through a sulfur or a selenium-containing bond to form a second peptide segment-resin, the second peptide segment comprising from about two to about one hundred amino acid residues;
- e. cleaving the second peptide segment-resin to form a second peptide segment having a thiol- or selenol-containing group at the C-terminus thereof;
- and
- f. coupling the haloacylpeptide segment and the second peptide segment via the thiol- or selenol-containing group to form a non-amido linkage.
4 more elements
Dependencies are inferred from the claim text.