Stabilization of bilirubin in control sera and calibrators
AssigneeBohringer Mannheim GmbH
InventorsGeoffrey Burns, Martina Junius-Comer
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Abstract
The invention concerns a stable control serum or calibration serum containing bilirubin to analytically examine the methodical accuracy of individual parameters in human sera or patients. The control serum or calibration serum according to the invention is used to stabilize bilirubin in solutions and to generally increase the stability of control sera or calibration sera.
Claims13
Control serum or calibration serum comprising bilirubin and a stabilizer comprising at least one compound selected from the group consisting of:
- a compound of formula (I):
- ##STR3## wherein R.sub.1 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.2 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.3 is H or OH;
- R.sub.4 is H, COOR.sub.5, COR.sub.5 or SO.sub.3 R.sub.5
- R.sub.5 is H or C.sub.1-6 alkyl;
- and a compound of formula (II) ##STR4## wherein R.sub.1 is H, Halogen or C.sub.1-6 alkyl;
- R2is H, C.sub.6 alkyl, or C.sub.1-6 alkoxy.
A process for the stabilization of a control serum or calibration serum containing bilirubin, said process comprising adding at least one compound selected from the group consisting of:
- a compound of formula (I) and a compound of formula (II) to a control serum or calibration serum containing bilirubin, wherein
- a compound of formula (I) is ##STR5## wherein R.sub.1 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.2 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.3 is or OH;
- R.sub.4 is H, COOR.sub.5, COR.sub.5 or SO.sub.3 R.sub.5
- R.sub.5 is H or C.sub.1-6, alkyl;
- and a compound of formula (II) is ##STR6## wherein R.sub.1 is H, Halogen or C.sub.1-6 alkyl;
- R.sub.2 is H, C.sub.1-6 alkyl , or C.sub.1-6 alkoxy.
A method for increasing the storage stability of control sera or calibration sera comprising bilirubin, comprising adding at least one compound selected from the group consisting of a compound of formula (I) and a compound of formula (II) to the control sera or calibration sera, wherein a compound of formula (I) is ##STR7## wherein R.sub.1 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.2 is H, OH, C.sub.1-8 alkyl, COOR.sub.5 or SO.sub.3 R.sub.5 ;
- R.sub.3 is H or OH;
- R.sub.4 is H, COOR.sub.5, COR.sub.5 or SO.sub.3 R.sub.5
- R.sub.5 is H or C.sub.1-6 alkyl;
- and a compound of formula (II) is ##STR8## wherein R.sub.1 is H, Halogen or C.sub.1-6 alkyl;
- R.sub.2 is H, C.sub.1-6 alkyl, or C.sub.1-6 alkoxy.
Dependencies are inferred from the claim text.