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AssigneeMount Sinai School of Medicine of the City University of New York
InventorsPeter Palese, Adolfo Garcia-Sastre
Estimating the term…
Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template. Bicistronic mRNAs can be constructed to permit internal initiation of translation of viral sequences and allow for the expression of foreign protein coding sequences from the regular terminal initiation site, or vice versa.
A recombinant RNA molecule comprising a binding site specific for an RNA-directed RNA polymerase of a negative strand RNA virus, operatively linked to a heterologous RNA sequence comprising the reverse complement of a bicistronic mRNA coding sequence containing an internal sequence that mediates internal initiation of translation.
A recombinant RNA molecule comprising a heterologous RNA sequence comprising the reverse complement of a bicistronic mRNA coding sequence containing an internal sequence that mediates internal initiation of translation, operatively linked to the 3'-noncoding flanking sequence of an influenza genomic RNA segment containing a viral polymerase binding site and operatively linked to the 5'-noncoding flanking sequence of an influenza genomic RNA sequence.
A chimeric virus comprising an influenza virus containing a heterologous RNA sequence comprising the reverse complement of a bicistronic mRNA coding sequence containing an internal sequence that mediates internal initiation of translation, operatively linked to an influenza viral polymerase binding site.
A chimeric virus comprising an influenza virus containing in addition to its eight genomic segments, an additional RNA segment containing a heterologous RNA sequence comprising the reverse complement of a bicistronic mRNA coding sequence containing an internal sequence that mediates internal initiation of translation, operatively linked to an influenza viral polymerase binding site.
A chimeric virus comprising a negative-strand RNA virus containing a heterologous RNA sequence comprising the reverse complement of a bicistronic mRNA coding sequence containing an internal sequence that mediates internal initiation of translation, operatively linked to a polymerase binding site of the negative-strand RNA virus.
Dependencies are inferred from the claim text.