Human growth hormone variant having enhanced affinity for human growth hormone receptor at site 1
AssigneeGenentech, Inc.
InventorsBrian C. Cunningham, James A. Wells
Life of this patent
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Abstract
A human growth hormone variant including the set of amino acid substitutions H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A is disclosed. Also disclosed is a nucleic acid encoding this variant, along with a vector including the nucleic acid, a host cell including the vector, and a process for preparing the variant. The variant has enhanced affinity for human growth hormone receptor at site 1.
Claims6
An isolated variant of a naturally occurring human growth hormone having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions:
- H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A.
An isolated nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions:
- H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A.
A vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions:
- H18A, Q22A, F26A, D26A, Q29A, E65A, K168A, E174A.
A host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions:
- H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A.
A process for preparing a human growth hormone variant comprising:
- (a) culturing a host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions:
- H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A;
- and
- (b) recovering the variant from the culture.
Dependencies are inferred from the claim text.