Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor
OwnerMichael S. Denison, Abraham Brouwer +1inventors
InventorsMichael S. Denison, Abraham Brouwer, George C. Clark
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Abstract
A recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286, also known as the mouse H1L1.1 cell line, has been made by using genetic engineering techniques for inserting dioxin responsive elements upstream of a luciferase reporter gene, and then, transfecting the resultant recombinant expression plasmid, identified as pGudLuc1.1, into mouse hepatoma cells. The mouse H1L1.1 cell line is useful in a method for the quantitative analysis of polyaromatic hydrocarbons in samples.
Claims31
A recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286 and known as the mouse H1L1.1c2 cell line, which will respond to selected polyaromatic hydrocarbon compounds with expression of luciferase activity.
A method for the quantitative analysis of selected polyaromatic hydrocarbon compounds in samples, the method comprising the steps of:
- (a) providing a sample containing a suspected amount of at least one of said polyaromatic hydrocarbon compounds;
- and
- (b) determining the amount of said polyaromatic hydrocarbon compound present in the sample by using a recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286 and known as the mouse H1L1.1c2 cell line.
A method for the quantitative analysis of selected polyaromatic hydrocarbon compounds in samples, the method comprising the steps of:
- (a) providing a sample containing a suspected amount of at least one of said polyaromatic hydrocarbon compounds;
- and
- (b) determining the amount of said polyaromatic hydrocarbon compound present in the sample by using a recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286 and known as the mouse H1L1.1c2 cell line, wherein the determining of the amount of said polyaromatic hydrocarbon compound present in the sample is accomplished in about 8 hours or less.
Dependencies are inferred from the claim text.