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US 5,853,996 AGranted
Detection of chronic fatigue syndrome by increased apoptosis and cell cycle arrest of peripheral blood mononuclear cells
AssigneeImmunosciences Lab, Inc.
InventorsEli Mordechai, Aristo Vojdani
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Abstract
A method for diagnosing chronic fatigue syndrome in an individual. Peripheral blood monocytes are isolated and p68 kinase activity, mRNA levels, protein levels, apoptosis and cell cycle analysis are measured. Significantly increased levels of any of these compared to healthy control individuals indicates the presence of chronic fatigue syndrome.
Claims3
A method of detecting chronic fatigue syndrome (CFS) in an individual, comprising the steps of:
- isolating peripheral blood mononuclear cells (PBMCs) from said individual;
- determining the number of apoptotic PBMCs present in PBMCs from said individual by fluorescently or radioactively labeling DNA strand breaks in said PBMCs and detecting said labeled strand breaks by flow cytometry;
- and
- comparing said number of apoptotic PBMCs present in PBMCs from said individual to an expected amount of apoptotic PBMCs from a control individual known not to have CFS, wherein an increase in said number of apoptotic PBMCs in said individual compared to said amount of apoptotic PBMCs from said control individual indicates the presence of CFS.
1 more element
A method of detecting chronic fatigue syndrome (CFS) in an individual, comprising the steps of:
- isolating peripheral blood mononuclear cells (PBMCs) from said individual;
- determining the number of PBMCs from said individual arrested in the late S and G.sub.2 /M stages of the cell cycle;
- and
- comparing said number of arrested PBMCs from said individual to an expected number of arrested PBMCs from an individual known not to have CFS, wherein an increase in said number of arrested PBMCs in said individual compared to said number of arrested PBMCs from said control individual indicates the presence of CFS.
1 more element
Dependencies are inferred from the claim text.