Method of isolating strains of the Lymantria dispar nuclear polyhedrosis virus
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorJames M. Slavicek
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Abstract
A method of isolating a gypsy moth virus that maintains normal levels of viral occlusion when produced in an insect cell line is disclosed. This method comprises the steps of cycles of infecting gypsy moth larvae with a strain of gypsy moth virus of interest, harvesting the larvae to collect budded virus, infecting a cell line capable of propagation in a bioreactor and then harvesting the virus. One repeats the infection cycle until one has enriched for a gypsy moth virus strain with normal levels of occlusion.
Claims12
A method for isolating a LdMNPV strain that maintains normal levels of virion occlusion when produced in an insect cell line, comprising the steps of:
- (a) infecting gypsy moth larvae with a strain of LdMNPV,
- (b) harvesting the larvae to collect budded virus within the insect hemolymph,
- (c) infecting an insect cell line and propagating the cell line in a bioreactor with the budded virus preparation,
- (d) harvesting virus from the insect cell line,
- (e) repeating the infection cycle of steps (a)-(d) until one has enriched for a LdMNPV strain with normal levels of occlusion, and
- (f) confirming that the strain has at least normal levels of occlusion.
Dependencies are inferred from the claim text.