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US 5,853,974 AGranted
Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates
AssigneeChiron Corporation
InventorPatrick J. Sheridan
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Abstract
Methods and compositions for enhancing the chemiluminescence from a stable 1,2-dioxetane triggered to produce a chemiluminescence are disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described that employ these methods and compositions.
Claims24
A method for enhancing the chemiluminescence from a molecule that is triggerable to generate a chemiluminescent signal, which comprises:
- (a) providing a molecule that is triggerable to generate a chemiluminescent signal, a dicationic surfactant, and a hydrophobic anionic enhancer having the formula R.sup.1 X.sup.- A.sup.+, wherein R.sup.1 is a hydrophobic group that may be a substituted or unsubstituted hydrocarbon moiety selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl, aryl and aralkyl, X.sup.- is an anionic moiety covalently attached to the R.sup.1 moiety, and A.sup.+ is a countercation;
- and
- (b) activating the triggerable molecule to generate a chemiluminescent signal.
A composition comprising:
- (a) a molecule that is triggerable to generate a chemiluminescent signal;
- (b) a dicationic surfactant;
- and
- (c) a hydrophobic anionic enhancer having the formula R.sup.1 X.sup.- A.sup.+, wherein R.sup.1 is a hydrophobic group that may be a substituted or unsubstituted hydrocarbon moiety selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl, aryl and aralkyl, X.sup.- is an anionic moiety covalently attached to the R.sup.1 moiety, and A.sup.+ is a countercation.
1 more element
Dependencies are inferred from the claim text.