Solid-phase method for attaching a biomolecule to a substrate surface with a photoreactive crosslinking agent

A method for making a medical device comprising a biomaterial having a biomolecule thereon, the method comprising:

• (a) providing an immobilized biomolecule comprising a peptide having an N.sup..alpha. -terminus covalently attached to a support material;
• (b) attaching a photoreactive crosslinking agent to the immobilized peptide at the N.sup..alpha. -terminus of the peptide to form an immobilized photoreactive analog of the biomolecule;
• (c) removing the photoreactive analog of the biomolecule from the support material;
• and
• (d) attaching the photoreactive analog of the biomolecule to a biomaterial contained by a medical device;
• the attaching step comprising:
• (i) contacting the photoreactive analog of the biomolecule to the biomaterial;
• and
• (ii) exposing the photoreactive analog of the biomolecule to UV radiation.

A method of promoting cell adhesion to a biomaterial, the method comprising:

• (a) providing an immobilized adhesion-promoting peptide comprising an adhesion-promoting peptide having an N.sup..alpha. -terminus covalently attached to a support material;
• (b) attaching a photoreactive crosslinking agent comprising at least one photoreactive group to the immobilized peptide at the N.sup..alpha. -terminus of the peptide to form an immobilized photoreactive analog of the adhesion-promoting peptide;
• (c) removing the photoreactive analog of the adhesion-promoting peptide from the support material;
• (d) attaching the photoreactive analog of the adhesion-promoting peptide to a biomaterial by activating the photoreactive group of the photoreactive analog;
• and
• (e) contacting cells with the biomaterial having the adhesion-promoting peptide thereon.

A solid-phase method of modifying a substrate surface to include a biomolecule, the method comprising:

• (a) providing an immobilized biomolecule comprising a peptide having an active site covalently attached to a support material;
• (b) attaching a photoreactive crosslinking agent comprising at least one photoreactive group to the immobilized biomolecule to form an immobilized photoreactive analog of the biomolecule, wherein the photoreactive crosslinking agent is coupled to the peptide at an amino acid group that is not part of the peptide's active site;
• (c) removing the photoreactive analog of the biomolecule from the support material;
• and
• (d) attaching the photoreactive analog of the biomolecule to a substrate surface by activating the photoreactive group of the photoreactive analog.

A method for making a medical device comprising a biomaterial having a biomolecule thereon, the method comprising:

• (a) providing an immobilized biomolecule comprising a peptide having an active site covalently attached to a support material;
• (b) attaching a photoreactive crosslinking agent to the immobilized biomolecule to form an immobilized photoreactive analog of the biomolecide, wherein the photoreactive crosslinking agent is coupled to the peptide at an amino acid group that is not part of the peptide's active site;
• (c) removing the photoreactive analog of the biomolecule from the support material;
• and
• (d) attaching the photoreactive analog of the biomolecule to a biomaterial contained by a medical device;
• the attaching step comprising:
• (i) contacting the photoreactive analog of the biomolecule to the biomaterial;
• and
• (ii) exposing the photoreactive analog of the biomolecule to UV radiation.

A method of promoting cell adhesion to a biomaterial, the method comprising:

• (a) providing an immobilized adhesion-promoting peptide comprising an adhesion-promoting peptide having an active site covalently attached to a support material;
• (b) attaching a photoreactive crosslinking agent comprising at least one photoreactive group to the immobilized adhesion-promoting peptide to form an immobilized photoreactive analog of the adhesion-promoting peptide, wherein the photoreactive crosslinking agent is coupled to the adhesion-promoting peptide at an amino acid group that is not part of the peptide's active site;
• (c) removing the photoreactive analog of the adhesion-promoting peptide from the support material;
• (d) attaching the photoreactive analog of the adhesion-promoting peptide to a biomaterial by activating the photoreactive group of the photoreactive analog;
• and
• (e) contacting cells with the biomaterial having the adhesion-promoting peptide thereon.