US 5,853,717 A · Methods and compositions of growth control for cells encapsulated within bioartificial organs

Abstract

This invention relates to methods and compositions of controlling cell distribution within a bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibiting compound; exposing the cells to irradiation, and (3) modifying a growth surface of the BAO with ECM molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination.

Claims14

Dependent claims

A method for controlling distribution of cells within a bioartificial organ comprising:

(a) providing a biocompatible, permselective jacket defining an internal volume;
(b) forming an inert scaffold within the internal volume defined by the jacket, wherein the inert scaffold is formed from a compound selected from the group consisting of poly(hydroxyethyl methacrylate) and poly(hydroxethyl methacrylate-co-methyl methacrylate);
and
(c) loading cells within the internal volume defined by the jacket, wherein the inert scaffold provides a structure for adhering and distributing the cells within the bioartificial organ.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

08/447,356Filed 23 May 1995 · division

Priority

20 Jul 1994

Granted

29 Dec 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification20 codes

C12N 5/0068
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: general…
A61L 27/18
Macromolecular materials: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
A61L 27/227
Polypeptides or derivatives thereof , e.g. degradation products: other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or…
A61L 27/383
Characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells: nerve cells, e.g. dendritic…

Methods and compositions of growth control for cells encapsulated within bioartificial organs

Life of this patent

Methods and compositions of growth control for cells encapsulated within bioartificial organs

Life of this patent

Abstract

Claims14