12,13,17-trihydroxy-9(z)-octadeoenoic acid and derivatives and microbial isolate for production of the acid
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorChing T. Hou
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Abstract
A novel compound, 12,13,17-trihydroxy-9(Z)-octadecenoic acid (THOA) was produced from linoleic acid by microbial transformation at 25% yield. The newly isolated microbial strain catalyzing this transformation was identified as Clavibacter sp. ALA2 (Accession No. NRRL B-21660). THOA and its derivatives have application as antifungal agents.
Claims6
A compound having the formula:
- ##STR2## wherein:
- R is --(O).sub.n --R.sub.1,
- n is 0,1, and
- R.sub.1 is H, or a hydrocarbon selected from the group consisting of substituted or unsubstituted alkyl, phenyl, or alkyl phenyl hydrocarbons, wherein the alkyl moiety may be branched or straight chain;
- and
- wherein:
- X.sub.1, X.sub.2, and X.sub.3 are independently selected from hydroxyl, halogen, or NR.sub.2 R.sub.3, wherein R.sub.2 and R.sub.3 are independently selected from the group consisting of hydrogen, substituted or unsubstituted alkyl, phenyl, or alkyl phenyl hydrocarbons, wherein the alkyl moiety may be branched or straight chain.
A method for converting linoleic acid to 12,13,17-trihydroxy-9(Z)-octadecenoic acid comprising cultivating a Clavibacter isolate having all the identifying characteristics of Accession No. NRRL B-21660 on a linoleic acid-containing substrate under conditions suitable for formation of said 12,13,17-trihydroxy-9(Z)-octadecenoic acid.
A Clavibacter isolate having all the identifying characteristics of Accession No. NRRL B-21660, including the property of converting linoleic acid to 12,13,17-trihydroxy-9(Z)-octadecenoic acid when said isolate is cultivated on a medium containing linoleic acid at a pH within the range of about 6.5 to 8 and at a temperature within the range of about 25.degree. C. to 40.degree. C.
Dependencies are inferred from the claim text.