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AssigneeJuridical Foundation The Chemo-Sero-Therapeutic Research Institute
InventorsHiroaki Maeda, Yasuyuki Eda, Kazuhiko Kimachi, Yoichi Ono, Sachio Tokiyoshi
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A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin wherein the latter gene fragment; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.
A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of a canine immunoglobulin lambda chain, wherein the amino acid sequence is selected from the group consisting of an amino acid sequence such that the 8th and 7th amino acid residues at the N-terminal region of the first cysteine counted from the N-terminus of a polypeptide of the constant region of said lamda chain are glycine and alanine, respectively, an amino acid sequence such that the 11th, 10th and 9th amino acid residues at the N-terminal region of the second cysteine counted from the C-terminus of the polypeptide of the constant region of said lambda chain are proline, aspartic acid and lysine, respectively, or both.
A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of a canine immunoglobulin kappa chain wherein the constant region comprises a polypeptide having 109 amino acids and the amino acid sequence of five amino acids at the C-terminus is SEQ ID NO:5.
A recombinant DNA molecule coding for an amino acid sequence of an L chain of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin L chain and a gene fragment coding for an amino acid sequence of a constant region of canine immunoglobulin L chain wherein the latter gene fragment is linked to 3' site of the former gene fragment, wherein the gene fragment coding for the amino acid sequence of the constant region of canine immunoglobulin L chain is a gene fragment coding for the amino acid sequence of the constant region of a canine immunoglobulin kappa chain which comprises a polypeptide having 109 amino acid and the amino acid sequence of five amino acids at the C-terminus is SEO ID NO:5.
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