Antibodies to liver derived receptors for advanced glycosylation endproducts and uses therefor
AssigneeThe Rockefeller University
InventorsHelen Vlassara, Zhi Yang, Anthony Cerami
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Abstract
The present invention relates to receptors for advanced glycosylation endproducts derived from rat liver membranes, and that specifically comprise proteins determined to possess molecular masses of about 90 kD and 60 kD, respectively, as assessed by migration during SDS-PAGE. Partial N-terminal sequences have been determined and diagnostic and therapeutic agents, compositions and methods are proposed. Antibodies to the 90 kD and 60 kD receptor proteins are disclosed.
Claims10
An isolated antibody that binds a receptor protein, said receptor protein to which said antibody is raised comprising a protein in purified form derived from rat liver cell membranes, that recognizes and binds advanced glycosylation endproducts (AGE) and that possesses the following characteristics:
- A. said protein recognizes and binds with the ligands AGE-RNase and AGE-Collagen I;
- B. said protein does not recognize and bind with the ligands FFI-BSA, formaldehyde-treated BSA, glucosamide-BSA, and acetyl LDL-BSA in solid phase ligand blotting assay;
- and
- C. said protein has a molecular mass of about 90 kD or about 60 kD as determined by protein migration on SDS-PAGE.
Dependencies are inferred from the claim text.