TNF receptor death ligand proteins and inhibitors of ligand binding
AssigneeGenetics Institute, Inc.
InventorsLih-Ling Lin, Jennifer Chen, Andrea R. Schievella, James Graham
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Abstract
Novel TNF receptor death domain ("TNF-R1-DD") ligand proteins are disclosed. Polynucleotides encoding the TNF-R1-DD ligand protein are also disclosed, along with vectors, host cells, and methods of making the TNF-R1-DD ligand protein. Pharmaceutical compositions containing the TNF-R1-DD ligand protein, methods of treating inflammatory conditions, and methods of inhibiting TNF-R death domain binding are also disclosed. Methods of identifying inhibitors of TNF-R death domain binding and inhibitors identified by such methods are also disclosed.
Claims7
A composition comprising a protein having TNF-R1-DD ligand protein activity wherein said protein comprises an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:4;
- and
- (b) fragments of the amino acid sequence of SEQ ID NO:4;
- said protein being substantially free from other mammalian proteins.
A composition comprising a protein having TNF-R1-DD ligand protein activity wherein said protein comprises an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:14;
- and
- (b) fragments of the amino acid sequence of SEQ ID NO:14;
- said protein being substantially free from other mammalian proteins.
TNF-R1-DD ligand protein produced according to a method comprising:
- (a) transforming a host cell with a composition comprising an isolated polynucleotide operably linked to an expression control sequence and encoding a TNF-R1-DD ligand protein comprising the amino acid sequence of SEQ ID NO:14;
- (b) growing a culture of the host cell in a suitable culture medium;
- and
- (c) purifying the TNF-R1-DD ligand protein from the culture.
Dependencies are inferred from the claim text.