bFGF mutein and its production
AssigneeTakeda Chemical Industries, Ltd.
InventorsAkira Fujishima, Tsunehiko Fukuda
Life of this patent
Estimating the term…
Abstract
A mutein resulting from substitution of cysteins for at least one of the constitutional amino acids has a high stability, and can serve well as a pharmaceutical, such as a healing promoting agent for wounds.
Claims13
A mutein of basic fibroblast qrowth factor (bFGF) wherein at least one amino acid is replaced with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 20 and 52,
- (b) amino acids 21 and 142,
- (c) amino acids 27 and 135,
- (d) amino acids 33 and 50,
- (e) amino acids 49 and 69 or
- (f) amino acids 81 and 126,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11) and wherein scold mutein possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
A mutein of basic fibroblast growth Factor (bFGF) wherein at least one amino acid is replaced with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 25 and 118 or
- (b) amino acids 25 and 139,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11) and wherein said mutein possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
A mutein of basic fibroblast growth factor (bFGF) wherein at least one amino acid is replaced with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 75 and 92 or
- (b) amino acids 85 and 92,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11)and wherein said mutein possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
A method for increasing stability of basic fibroblast growth factor (bFGF) comprising replacing at least one amino acid with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 20 and 52,
- (b) amino acids 21 and 142,
- (c) amino acids 27 an 135,
- (d) amino acids 33 and 50,
- (e) amino acids 49 and 69 or
- (f) amino acids 81 and 126,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11) and wherein the mutated bFGF possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
A method for increasing stability of basic fibroblast growth factor (bFGF) comprising replacing at least one amino acid with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 25 and 118 or
- (b) amino acids 25 and 139,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11)and wherein the mutated bFGF possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
A method for increasing stability of basic fibroblast growth factor (bFGF) comprising replacing at least one amino acid with a cysteine residue so as to form an intramolecular disulfide bond between
- (a) amino acids 75 and 92 or
- (b) amino acids 85 and 92,
- wherein the amino acid positions refer to the amino acid sequence of FIG. 1 (SEQ ID NO:
- 11) and wherein the mutated bFGF possesses increased stability under acidic conditions or increased temperature as compared to native bFGF.
Dependencies are inferred from the claim text.