Cultured high-fidelity three-dimensional human urogenital tract carcinomas and process
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Administration
InventorsThomas J. Goodwin, Tacey L. Prewett, Glenn F. Spaulding, David A. Wolf
Life of this patent
Estimating the term…
Abstract
Artificial high-fidelity three-dimensional human urogenital tract carcinomas are propagated under in vitro-microgravity conditions from carcinoma cells. Artificial high-fidelity three-dimensional human urogenital tract carcinomas are also propagated from a coculture of normal urogenital tract cells inoculated with carcinoma cells. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.
Claims25
A process for producing aggregates of urogenital tract carcinoma cells comprising the steps of
- (a) inoculating urogenital tract carcinoma cells into a culture vessel containing culture media and a culture matrix;
- (b) culturing the urogenital tract carcinoma cells under microgravity conditions whereby the urogenital tract carcinoma cells form aggregates;
- and
- (c) maintaining the microgravity culture conditions whereby high-fidelity, three-dimensional urogenital tract carcinoma growth is achieved.
A process for producing aggregates of urogenital tract carcinoma cells comprising the steps of
- (a) inoculating normal mammalian cells into a culture vessel containing culture media and a culture matrix;
- (b) inoculating urogenital tract carcinoma cells into the culture vessel;
- (c) co-culturing the normal mammalian cells and the urogenital tract carcinoma cells under microgravity conditions;
- and
- (d) maintaining the microgravity culture conditions whereby high-fidelity, three-dimensional urogenital tract carcinoma growth is achieved.
Dependencies are inferred from the claim text.