IKK-.beta. proteins, nucleic acids and methods
AssigneeTularik Inc.
InventorsDavid V. Goeddel, John Woronicz
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Abstract
The invention provides methods and compositions relating to an I.kappa.B kinase, IKK-.beta., and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed IKK-.beta. encoding nucleic acids or purified from human cells. The invention provides isolated IKK-.beta. hybridization probes and primers capable of specifically hybridizing with the disclosed IKK-.beta. genes, IKK-.beta.-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.
Claims12
An isolated or recombinant nucleic acid comprising at least 96 consecutive nucleotides of SEQ ID NO:
- 1.
An isolated or recombinant nucleic acid encoding a polypeptide comprising at least 31 consecutive residues of the amino acid sequence set forth as SEQ ID NO:
- 2.
A method of making an isolated polypeptide, said method comprising the steps of:
- introducing the recombinant nucleic acid encoding a polypeptide comprising at least 11 consecutive residues of the amino acid sequence set forth as SEQ ID NO:
- 2 into a host cell or cellular extract,
- incubating said host cell or cellular extract under conditions whereby said polypeptide is expressed;
- and
- isolating said polypeptide.
Dependencies are inferred from the claim text.