Peroxidase variants with improved hydrogen peroxide stability
AssigneeNovo Nordisk A/S
InventorsKaren Gjesing Welinder, Morten Birket Andersen
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Abstract
It has surprisingly been found that peroxidase variants with an improved stability towards hydrogen peroxide may be prepared by recombinant DNA techniques. Accordingly, the present invention relates to a peroxidase variant with improved hydrogen peroxide stability, characterized by insertion, deletion or substitution of one or more amino acid residues located in or near the substrate channel of the parent peroxidase, near the heme group of the parent peroxidase or at or near the active site of the parent peroxidase.
Claims20
A variant of a Coprinus cinereus peroxidase, comprising one or more substitutions selected from the group consisting of:
- Q1S, E,
- S8N,
- C22S,
- C23S,
- Q38N,
- D56N, T, I,
- N142S, T, P, D,
- G154E,
- G156F,
- N157E, F,
- D245I, T, N,
- C256S,
- S263N,
- N265S,
- A304S,
- T331A, N, and
- S338A,
- wherein the peroxidase has an amino acid sequence of SEQ ID NO:
- 2 and the variant has improved hydrogen peroxide stability over the peroxidase.
Dependencies are inferred from the claim text.