Tryptophanyl tRNA Synthetase polynucleotides of Streptococcus

An isolated polynucleotide comprising the polynucleotide sequence of SEQ ID NO:

• 1.

An isolated polynucleotide consisting of the polynucleotide sequence of SEQ ID NO. 1.

An isolated polynucleotide comprising a DNA sequence or an RNA sequence obtained by screening an appropriate library containing the complete gene encoding an amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a probe having a polynucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2 or a fragment thereof, which fragment retains either or both of binding or catalytic activity;

• and isolating said DNA sequence or RNA sequence, which DNA sequence or RNA sequence hybridizes over the entire length of said probe.

An isolated polynucleotide sequence of SEQ ID NO:

• 1 with up to five point mutations per each 100 nucleotides of the reference nucleotide sequence of SEQ ID NO:
• 1, which mutations may be deletions or substitutions with another nucleotide, or a number of nucleotides up to 5% of the total nucleotides in the reference sequence may be inserted into the reference sequence, or may occur at the 5' or 3' terminal positions of the reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among aucleotides in the reference sequence or in one or more contiguous groups within the reference sequence.

An isolated polynucleotide comprising a polynucleotide encoding a polypeptide of SEQ ID NO:

• 2 with up to five amino acid alterations per each 100 amino acids of the reference amino acid of SEQ ID NO:
• 2, or up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or a number of amino acids up to 5% of the total amino acid residues in the reference sequence may be inserted into the reference sequence, and these alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.

A probe comprising an isolated polynucleotide selected from the group consisting of:

• at least 30 bases of the polynucleotide sequence set forth in SEQ ID NO:
• 1 and at least 50 bases of the polynucleotide sequence set forth in SEQ ID NO:
• 1.

A probe consisting of an isolated polynucleotide selected from the group consisting of:

• at least 30 bases of the polynucleotide sequence set forth in SEQ ID NO:
• 1 and at least 50 bases of the polynucleotide sequence set forth in SEQ ID NO:
• 1.