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US 5,851,794 AGranted
Collagen binding protein as well as its preparation
AssigneeAlfa-Laval AB
InventorsBengt Guss, Magnus Hook, Hans Jonsson, Martin Lindberg, Joseph Patti, Christer Signas, Lech Switalski
Life of this patent
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Abstract
The present invention relates to a new recombinant DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having collagen binding properties.
Claims9
A plasmid pSAC104 as contained in the E. coli TG1 having the deposit number DSM 6199.
An isolated DNA-molecule characterized in that it comprises the following nucleotide sequence SEQ ID NO:
- 1!:
- ATGCACTTGT ATTCGTTATA CTGTATATAT TTTGCATAAT AAAATAATAA TATGAATTTT
- TGATAAATTT CATTGAATAA GAACTAAATT AGTTTATAAT TTATTATTAG TATCCTGTGG
- ATATGACATA GAGTATAAGG AGGGGTTTTT ATGAACAAAA ATGTGTTGAA GTTTATGGTC
- TTTATAATGT TATTAAATAT CATCACACCT TTATTTAATA AAAATGAAGC ATTTGCAGCA
- CGAGATATTT CATCAACGAA TGTTACAGAT TTAACTGTAT CACCGTCTAA GATAGAAGAT
- GGTGGTAAAA CGACAGTAAA AATGACGTTC GACGATAAAA ATGGAAAAAT ACAAAATGGT
- GACATGATTA AAGTGGCATG GCCGACAAGC GGTACAGTAA AGATAGAGGG TTATAGTAAA
- ACAGTACCAT TAACTGTTAA AGGTGAACAG GTGGGTCAAG CAGTTATTAC ACCAGACGGT
- GCAACAATTA CATTCAATGA TAAAGTAGAA AAATTAAGTG ATGTTTCGGG ATTTGCAGAA
- TTTGAAGTAC AAGGAAGAAA TTTAACGCAA ACAAATACTT CAGATGACAA AGTAGCTACG
- ATAACATCTG GGAATAAATC AACGAATGTT ACGGTTCATA AAAGTGAAGC GGGAACAAGT
- AGTGTTTTCT ATTATAAAAC GGGAGATATG CTACCAGAAG ATACGACACA TGTACGATGG
- TTTTTAAATA TTAACAATGA AAAAAGTTAT GTATCGAAAG ATATTACTAT AAAGGATCAG
- ATTCAAGGTG GACAGCAGTT AGATTTAAGC ACATTAAACA TTAATGTGAC AGGTACACAT
- AGCAATTATT ATAGTGGACA AAGTGCAATT ACTGATTTTG AAAAAGCCTT TCCAGGTTCT
- AAAATAACTG TTGATAATAC GAAGAACACA ATTGATGTAA CAATTCCACA AGGCTATGGG
- TCATATAATA GTTTTTCAAT TAACTACAAA ACCAAAATTA CGAATGAACA GCAAAAAGAG
- TTTGTTAATA ATTCACAAGC TTGGTATCAA GAGCATGGTA AGGAAGAAGT GAACGGGAAA
- TCATTTAATC ATACTGTGCA CAATATTAAT GCTAATGCCG GTATTGAAGG TACTGTAAAA
- GGTGAATTAA AAGTTTTAAA ACAGGATAAA GATACCAAGG CTCCTATAGC TAATGTAAAA
- TTTAAACTTT CTAAAAAAGA TGGATCAGTT GTAAAGGACA ATCAAAAAGA AATTGAGATT
- ATAACAGATG CAAACGGTAT TGCTAATATT AAAGCGTTGC CTAGTGGAGA CTATATTTTA
- AAAGAAATAG AGGCGCCACG ACCGTATACA TTTGATAAGG ATAAAGAATA TCCGTTTACT
- ATGAAAGATA CAGATAATCA GGGATATTTT ACGACTATTG AAAATGCAAA AGCGATAGAA
- AAAACAAAAG ATGTTTCTGC TCAAAAGGTT TGGGAAGGCA CTCAAAAAGT GAAACCAACG
- ATTTATTTCA AGTTGTACAA ACAAGATGAC AATCAAAATA CAACACCAGT AGACAAAGCA
- GAGATTAAAA AATTAGAAGA TGGAACGACA AAAGTGACAT GGTCTAATCT TCCGGAAAAT
- GACAAAAATG GCAAGGCTAT TAAATATTTA GTTAAAGAAG TAAATGCTCA AGGTGAAGAT
- ACAACACCAG AAGGATATAC TAAAAAAGAA AATGGTTTAG TGGTTACTAA TACTGAAAAA
- CCAATCGAAA CAACATCAAT TAGTGGTGAA AAAGTATGGG ACGACAAAGA CAATCAAGAT
- GGTAAGAGAC CAGAAAAAGT CAGTGTGAAT TTATTGGCTA ACGGGGAGAA AGTAAAAACG
- TTAGACGTGA CATCTGAAAC AAACTGGAAG TACGAATTTA AAGACTTACC GAAGTATGAT
- GAAGGAAAGA AAATAGAATA TACAGTGACC GAAGATCACG TAAAAGACTA CACAACAGAC
- ATCAACGGTA CGACAATAAC GAACAAGTAT ACACCAGGAG AGACATCGGC AACAGTAACA
- AAAAATTGGG ATGACAATAA TAACCAAGAC GGAAAACGAC CAACTGAAAT CAAAGTTGAG
- TTATATCAAG ATGGAAAAGC AACAGGAAAA ACGGCAATAT TAAATGAATC TAATAACTGG
- ACACATACGT GGACAGGATT AGATGAAAAA GCAAAAGGAC AACAAGTAAA ATACACAGTC
- GAGGAATTAA CAAAGGTCAA AGGTTATACA ACACATGTGG ATAACAATGA TATGGGTAAC
- TTGATTGTGA CGAATAAATA TACGCCAGAA ACAACATCAA TTAGTGGTGA AAAAGTATGG
- GACGACAAAG ACAATCAAGA TGGTAAGAGA CCAGAAAAAG TCAGTGTGAA TTTATTGGCT
- GATGGAGAGA AAGTAAAAAC GTTAGACGTG ACATCTGAAA CAAACTGGAA GTACGAATTT
- AAAGACTTAC CGAAGTATGA TGAAGGAAAG AAAATAGAAT ATACAGTGAC CGAAGATCAC
- GTAAAAGACT ACACAACAGA CATCAACGGT ACGACAATAA CGAACAAGTA TACACCAGGA
- GAGACATCGG CAACAGTAAC AAAAAATTGG GATGACAATA ATAACCAAGA CGGAAAACGA
- CCAACTGAAA TCAAAGTTGA GTTATATCAA GATGGAAAAG CAACAGGAAA AACGGCAATA
- TTAAATGAAT CTAATAACTG GACACATACG TGGACAGGAT TAGATGAAAA AGCAAAAGGA
- CAACAAGTAA AATACACAGT CGAGGAATTA ACAAAGGTCA AAGGTTATAC AACACATGTG
- GATAACAATG ATATGGGCAA CTTGATTGTG ACGAATAAAT ATACGCCAGA AACAACATCA
- ATTAGTGGTG AAAAAGTATG GGACGACAAA GACAATCAAG ATGGTAAGAG ACCAGAAAAA
- GTCAGTGTGA ATTTATTGGC TAACGGAGAG AAAGTAAAAA CGTTAGACGT GACATCTGAA
- ACAAACTGGA AGTACGAATT TAAAGACTTA CCGAAGTATG ATGAAGGAAA GAAAATAGAA
- TATACAGTGA CCGAAGATCA CGTAAAAGAC TACACAACAG ACATCAACGG TACGACAATA
- ACGAACAAGT ATACACCAGG AGAGACATCG GCAACAGTAA CAAAAAATTG GGATGACAAT
- AATAACCAAG ACGGAAAACG ACCAACTGAA ATCAAAGTTG AGTTATATCA AGATGGAAAA
- GCAACAGGAA AAACGGCAAT ATTAAATGAA TCTAATAACT GGACACATAC GTGGACAGGA
- TTAGATGAAA AAGCAAAAGG ACAACAAGTA AAATACACAG TCGATGAATT AACAAAAGTT
- AATGGCTATA CAACGCATGT GGATAACAAT GATATGGGTA ACTTGATTGT GACAAATAAA
- TATACGCCGA AAAAACCGAA TAAACCAATC TATCCTGAAA AACCAAAAGA CAAAACACCA
- CCAACTAAAC CTGATCATTC TAATAAAGTT AAACCAACTC CCCCAGATAA GCCATCAAAA
- GTGGATAAGG ATGATCAACC TAAAGATAAT AAAACCAAAC CTGAAAATCC TCTAAAAGAA
- TTACCAAAAA CTGGTATGAA GATTATAACT TCATGGATTA CATGGGTATT TATAGGTATA
- TTGGGACTGT ATTTAATTTT AAGAAAAAGA TTTAACTCAT AAACCATTAT AATTATTTTT
- ATAGATAAGG CTATTCTTAG TTCTATGTAT AATACATGTA TATTAATAGG TCACTTTTAA
- TCTGTATGTA AGCAGACTAA GAGTGGCCTT TTAAACAAAT AAAAAAA.
62 more elements
Dependencies are inferred from the claim text.