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US 5,851,793 AGranted
Recombinant DNA encoding receptor protein for human B-cell stimulatory factor-2
OwnerTadamitsu Kishimotoinventor
Life of this patent
Estimating the term…
Abstract
An isolated receptor protein for human B cell stimulatory factor-2, capable of specifically binding to the human B cell stimulatory factor-2; DNA coding for the above-mentioned receptor protein; expression vectors containing the above-mentioned DNA; host organisms transformed with the above-mentioned expression vector; a process for production of the receptor protein comprising culturing the host organisms in a medium to produce the receptor protein and recovering the receptor protein from the culture; and a antibody reacting with the protein.
Claims42
A DNA coding for a receptor protein consisting essentially of an active site for binding human B cell stimulating factor-2, wherein said active site is located between amino acid 123 and amino acid 323 in the following amino acid sequence:
- (I) (N-terminal)
- Met Leu Ala Val Gly Cys Ala Leu Leu Ala Ala
- Leu Leu Ala Ala Pro Gly Ala Ala Leu Ala Pro
- Arg Arg Cys Pro Ala Gln Glu Val Ala Arg Gly
- Val Leu Thr Ser Leu Pro Gly Asp Ser Val Thr
- Leu Thr Cys Pro Gly Val Glu Pro Glu Asp Asn
- Ala Thr Val His Trp Val Leu Arg Lys Pro Ala
- Ala Gly Ser His Pro Ser Arg Trp Ala Gly Met
- Gly Arg Arg Leu Leu Leu Arg Ser Val Gln Leu
- His Asp Ser Gly Asn Tyr Ser Cys Tyr Arg Ala
- Gly Arg Pro Ala Gly Thr Val His Leu Leu Val
- Asp Val Pro Pro Glu Glu Pro Gln Leu Ser Cys
- Phe Arg Lys Ser Pro Leu Ser Asn Val Val Cys
- Glu Trp Gly Pro Arg Ser Thr Pro Ser Leu Thr
- Thr Lys Ala Val Leu Leu Val Arg Lys Phe Gln
- Asn Ser Pro Ala Glu Asp Phe Gln Glu Pro Cys
- Gln Tyr Ser Gln Glu Ser Gln Lys Phe Ser Cys
- Gln Leu Ala Val Pro Glu Gly Asp Ser Ser Phe
- Tyr Ile Val Ser Met Cys Val Ala Ser Ser Val
- Gly Ser Lys Phe Ser Lys Thr Gln Thr Phe Gln
- Gly Cys Gly Ile Leu Gln Pro Asp Pro Pro Ala
- Asn Ile Thr Val Thr Ala Val Ala Arg Asn Pro
- Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe
- Glu Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr
- Phe Thr Thr Trp Met Val Lys Asp Leu Gln His
- His Cys Val Ile His Asp Ala Trp Ser Gly Leu
- Arg His Val Val Gln Leu Arg Ala Gln Glu Glu
- Phe Gly Gln Gly Glu Trp Ser Glu Trp Ser Pro
- Glu Ala Met Gly Thr Pro Trp Thr Glu Ser Arg
- Ser Pro Pro Ala Glu Asn Glu Val Ser Thr Pro
- Met Gln Ala Leu Thr Thr Asn Lys Asp Asp Asp
- Asn Ile Leu Phe Arg Asp Ser Ala Asn Ala Thr
- Ser Leu Pro Val Gln Asp Ser Ser Ser Val Pro
- Leu Pro Thr Phe Leu Val Ala Gly Gly Ser Leu
- Ala Phe Gly Thr Leu Leu Cys Ile Ala Ile Val
- Leu Arg Phe Lys Lys Thr Trp Lys Leu Arg Ala
- Leu Lys Glu Gly Lys Thr Ser Met His Pro Pro
- Tyr Ser Leu Gly Gln Leu Val Pro Glu Arg Pro
- Arg Pro Thr Pro Val Leu Val Pro Leu Ile Ser
- Pro Pro Val Ser Pro Ser Ser Leu Gly Ser Asp
- Asn Thr Ser Ser His Asn Arg Pro Asp Ala Arg
- Asp Pro Arg Ser Pro Tyr Asp Ile Ser Asn Thr
- Asp Tyr Phe Phe Pro Arg,
- (C-terminal).
42 more elements
A DNA coding for a receptor protein, wherein said receptor protein consists essentially of the amino acid sequence:
- Arg Lys Ser Pro Leu Ser Asn Val Val Cys Glu Trp
- Gly Pro Arg Ser Thr Pro Ser Leu Thr Thr Lys Ala
- Val Leu Leu Val Arg Lys Phe Gln Asn Ser Pro Ala
- Glu Asp Phe Gln Glu Pro Cys Gln Tyr Ser Gln Glu
- Ser Gln Lys Phe Ser Cys Gln Leu Ala Val Pro Glu
- Gly Asp Ser Ser Phe Tyr Ile Val Ser Met Cys Val
- Ala Ser Ser Val Gly Ser Lys Phe Ser Lys Thr Gln
- Thr Phe Gln Gly Cys Gly Ile Leu Gln Pro Asp Pro
- Pro Ala Asn Ile Thr Val Thr Ala Val Ala Arg Asn
- Pro Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe Glu
- Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr Phe Thr
- Thr Trp Met Val Lys Asp Leu Gln His His Cys Val
- Ile His Asp Ala Trp Ser Gly Leu Arg His Val Val
- Gln Leu Arg Ala Gln Glu Glu Phe Gly Gln Gly Glu
- Trp Ser Glu Trp Ser Pro Glu Ala Met Gly Thr Pro
- Trp Thr Glu Ser Arg Ser Pro Pro Ala.
14 more elements
A DNA coding for a human B cell stimulating factor-2 receptor protein consisting essentially of amino acid 1 to amino acid 323 in the following amino acid sequence:
- (I) (N-terminal)
- Met Leu Ala Val Gly Cys Ala Leu Leu Ala Ala
- Leu Leu Ala Ala Pro Gly Ala Ala Leu Ala Pro
- Arg Arg Cys Pro Ala Gln Glu Val Ala Arg Gly
- Val Leu Thr Ser Leu Pro Gly Asp Ser Val Thr
- Leu Thr Cys Pro Gly Val Glu Pro Glu Asp Asn
- Ala Thr Val His Trp Val Leu Arg Lys Pro Ala
- Ala Gly Ser His Pro Ser Arg Trp Ala Gly Met
- Gly Arg Arg Leu Leu Leu Arg Ser Val Gln Leu
- His Asp Ser Gly Asn Tyr Ser Cys Tyr Arg Ala
- Gly Arg Pro Ala Gly Thr Val His Leu Leu Val
- Asp Val Pro Pro Glu Glu Pro Gln Leu Ser Cys
- Phe Arg Lys Ser Pro Leu Ser Asn Val Val Cys
- Glu Trp Gly Pro Arg Ser Thr Pro Ser Leu Thr
- Thr Lys Ala Val Leu Leu Val Arg Lys Phe Gln
- Asn Ser Pro Ala Glu Asp Phe Gln Glu Pro Cys
- Gln Tyr Ser Gln Glu Ser Gln Lys Phe Ser Cys
- Gln Leu Ala Val Pro Glu Gly Asp Ser Ser Phe
- Tyr Ile Val Ser Met Cys Val Ala Ser Ser Val
- Gly Ser Lys Phe Ser Lys Thr Gln Thr Phe Gln
- Gly Cys Gly Ile Leu Gln Pro Asp Pro Pro Ala
- Asn Ile Thr Val Thr Ala Val Ala Arg Asn Pro
- Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe
- Glu Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr
- Phe Thr Thr Trp Met Val Lys Asp Leu Gln His
- His Cys Val Ile His Asp Ala Trp Ser Gly Leu
- Arg His Val Val Gln Leu Arg Ala Gln Glu Glu
- Phe Gly Gln Gly Glu Trp Ser Glu Trp Ser Pro
- Glu Ala Met Gly Thr Pro Trp Thr Glu Ser Arg
- Ser Pro Pro Ala Glu Asn Glu Val Ser Thr Pro
- Met Gln Ala Leu Thr Thr Asn Lys Asp Asp Asp
- Asn Ile Leu Phe Arg Asp Ser Ala Asn Ala Thr
- Ser Leu Pro Val Gln Asp Ser Ser Ser Val Pro
- Leu Pro Thr Phe Leu Val Ala Gly Gly Ser Leu
- Ala Phe Gly Thr Leu Leu Cys Ile Ala Ile Val
- Leu Arg Phe Lys Lys Thr Trp Lys Leu Arg Ala
- Leu Lys Glu Gly Lys Thr Ser Met His Pro Pro
- Tyr Ser Leu Gly Gln Leu Val Pro Glu Arg Pro
- Arg Pro Thr Pro Val Leu Val Pro Leu Ile Ser
- Pro Pro Val Ser Pro Ser Ser Leu Gly Ser Asp
- Asn Thr Ser Ser His Asn Arg Pro Asp Ala Arg
- Asp Pro Arg Ser Pro Tyr Asp Ile Ser Asn Thr
- Asp Tyr Phe Phe Pro Arg,
- (C-terminal).
42 more elements
A DNA coding for a human B cell stimulating factor-2 receptor protein consisting essentially of amino acid 1 to amino acid 344 in the following amino acid sequence:
- (I) (N-terminal)
- Met Leu Ala Val Gly Cys Ala Leu Leu Ala Ala
- Leu Leu Ala Ala Pro Gly Ala Ala Leu Ala Pro
- Arg Arg Cys Pro Ala Gln Glu Val Ala Arg Gly
- Val Leu Thr Ser Leu Pro Gly Asp Ser Val Thr
- Leu Thr Cys Pro Gly Val Glu Pro Glu Asp Asn
- Ala Thr Val His Trp Val Leu Arg Lys Pro Ala
- Ala Gly Ser His Pro Ser Arg Trp Ala Gly Met
- Gly Arg Arg Leu Leu Leu Arg Ser Val Gln Leu
- His Asp Ser Gly Asn Tyr Ser Cys Tyr Arg Ala
- Gly Arg Pro Ala Gly Thr Val His Leu Leu Val
- Asp Val Pro Pro Glu Glu Pro Gln Leu Ser Cys
- Phe Arg Lys Ser Pro Leu Ser Asn Val Val Cys
- Glu Trp Gly Pro Arg Ser Thr Pro Ser Leu Thr
- Thr Lys Ala Val Leu Leu Val Arg Lys Phe Gln
- Asn Ser Pro Ala Glu Asp Phe Gln Glu Pro Cys
- Gln Tyr Ser Gln Glu Ser Gln Lys Phe Ser Cys
- Gln Leu Ala Val Pro Glu Gly Asp Ser Ser Phe
- Tyr Ile Val Ser Met Cys Val Ala Ser Ser Val
- Gly Ser Lys Phe Ser Lys Thr Gln Thr Phe Gln
- Gly Cys Gly Ile Leu Gln Pro Asp Pro Pro Ala
- Asn Ile Thr Val Thr Ala Val Ala Arg Asn Pro
- Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe
- Glu Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr
- Phe Thr Thr Trp Met Val Lys Asp Leu Gln His
- His Cys Val Ile His Asp Ala Trp Ser Gly Leu
- Arg His Val Val Gln Leu Arg Ala Gln Glu Glu
- Phe Gly Gln Gly Glu Trp Ser Glu Trp Ser Pro
- Glu Ala Met Gly Thr Pro Trp Thr Glu Ser Arg;
- Ser Pro Pro Ala Glu Asn Glu Val Ser Thr Pro
- Met Gln Ala Leu Thr Thr Asn Lys Asp Asp Asp
- Asn Ile Leu Phe Arg Asp Ser Ala Asn Ala Thr
- Ser Leu Pro Val Gln Asp Ser Ser Ser Val Pro
- Leu Pro Thr Phe Leu Val Ala Gly Gly Ser Leu
- Ala Phe Gly Thr Leu Leu Cys Ile Ala Ile Val
- Leu Arg Phe Lys Lys Thr Trp Lys Leu Arg Ala
- Leu Lys Glu Gly Lys Thr Ser Met His Pro Pro
- Tyr Ser Leu Gly Gln Leu Val Pro Glu Arg Pro
- Arg Pro-Thr Pro Val Leu Val Pro Leu Ile Ser
- Pro Pro Val Ser Pro Ser Ser Leu Gly Ser Asp
- Asn Thr Ser Ser His Asn Arg Pro Asp Ala Arg
- Asp Pro Arg Ser Pro Tyr Asp Ile Ser Asn Thr
- Asp Tyr Phe Phe Pro Arg,
- (C-terminal).
42 more elements
A DNA coding for a human B cell stimulating factor-2 receptor protein consisting essentially of amino acid (1) 1 to amino acid 27 fused to amino acid (2) 110 to amino acid 468 in the following amino acid sequence:
- (I) (N-terminal)
- Met Leu Ala Val Gly Cys Ala Leu Leu Ala Ala
- Leu Leu Ala Ala Pro Gly Ala Ala Leu Ala Pro
- Arg Arg Cys Pro Ala Gln Glu Val Ala Arg Gly
- Val Leu Tht Ser Leu Pro Gly Asp Ser Val Thr
- Leu Thr Cys Pro Gly Val Glu Pro Glu Asp Asn
- Ala Thr Val His Trp Val Leu Arg Lys Pro Ala
- Ala Gly Ser His Pro Ser Arg Trp Ala Gly Met
- Gly Arg Arg Leu Leu Leu Arg Ser Val Gln Leu
- His Asp Ser Gly Asn Tyr Ser Cys Tyr Arg Ala
- Gly Arg Pro Ala Gly Thr Val His Leu Leu Val
- Asp Val Pro Pro Glu Glu Pro Gln Leu Ser Cys
- Phe Arg Lys Ser Pro Leu Ser Asn Val Val Cys
- Glu Trp Gly Pro Arg Ser Thr Pro Ser Leu Thr
- Thr Lys Ala Val Leu Leu Val Arg Lys Phe Gln
- Asn Ser Pro Ala Glu Asp Phe Gln Glu Pro Cys
- Gln Tyr Ser Gln Glu Ser Gln Lys Phe Ser Cys
- Gln Leu Ala Val Pro Glu Gly Asp Ser Ser Phe
- Tyr Ile Val Ser Met Cys Val Ala Ser Ser Val
- Gly Ser Lys Phe Ser Lys Thr Gln Thr Phe Gln
- Gly Cys Gly Ile Leu Gln Pro Asp Pro Pro Ala
- Asn Ile Thr Val Thr Ala Val Ala Arg Asn Pro
- Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe
- Glu Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr
- Phe Thr Thr Trp Met Val Lys Asp Leu Gln His
- His Cys Val Ile His Asp Ala Trp Ser Gly Leu
- Arg His Val Val Gln Leu Arg Ala Gln Glu Glu
- Phe Gly Gln Gly Glu Trp Ser Glu Trp Ser Pro
- Glu Ala Met Gly Thr Pro Trp Thr Glu Ser Arg
- Ser Pro Pro Ala Glu Asn Glu Val Ser Thr Pro
- Met Gln Ala Leu Thr Thr Asn Lys Asp Asp Asp
- Asn Ile Leu Phe Arg Asp Ser Ala Asn Ala Thr
- Ser Leu Pro Val Gln Asp Ser Ser Ser Val Pro
- Leu Pro Thr Phe Leu Val Ala Gly Gly Ser Leu
- Ala Phe Gly Thr Leu Leu Cys Ile Ala Ile Val
- Leu Arg Phe Lys Lys Thr Trp Lys Leu Arg Ala
- Leu Lys Glu Gly Lys Thr Ser Met His Pro Pro
- Tyr Ser Leu Gly Gln Leu Val Pro Glu Arg Pro
- Arg Pro Thr Pro Val Leu Val Pro Leu Ile Ser
- Pro Pro Val Ser Pro Ser Ser Leu Gly Ser Asp
- Asn Thr Ser Ser His Asn Arg Pro Asp Ala Arg
- Asp Pro Arg Ser Pro Tyr Asp Ile Ser Asn Thr
- Asp Tyr Phe Phe Pro Arg,
- (C-terminal).
42 more elements
A DNA coding for a human B cell stimulating factor-2 receptor protein consisting essentially of amino acid 1 to amino acid 468 in the following amino acid sequence:
- (I) (N-terminal)
- Met Leu Ala Val Gly Cys Ala Leu Leu Ala Ala
- Leu Leu Ala Ala Pro Gly Ala Ala Leu Ala Pro
- Arg Arg Cys Pro Ala Gln Glu Val Ala Arg Gly
- Val Leu Thr Ser Leu Pro Gly Asp Ser Val Thr
- Leu Thr Cys Pro Gly Val Glu Pro Glu Asp Asn
- Ala Thr Val His Trp Val Leu Arg Lys Pro Ala
- Ala Gly Ser His Pro Ser Arg Trp Ala Gly Met
- Gly Arg Arg Leu Leu Leu Arg Ser Val Gln Leu
- His Asp Ser Gly Asn Tyr Ser Cys Tyr Arg Ala
- Gly Arg Pro Ala Gly Thr Val His Leu Leu Val
- Asp Val Pro Pro Glu Glu Pro Gln Leu Ser Cys
- Phe Arg Lys Ser Pro Leu Ser Asn Val Val Cys
- Glu Trp Gly Pro Arg Ser Thr Pro Ser Leu Thr
- Thr Lys Ala Val Leu Leu Val Arg Lys Phe Gln
- Asn Ser Pro Ala Glu Asp Phe Gln Glu Pro Cys
- Gln Tyr Ser Gln Glu Ser Gln Lys Phe Ser Cys
- Gln Leu Ala Val Pro Glu Gly Asp Ser Ser Phe
- Tyr Ile Val Ser Met Cys Val Ala Ser Ser Val
- Gly Ser Lys Phe Ser Lys Thr Gln Thr Phe Gln
- Gly Cys Gly Ile Leu Gln Pro Asp Pro Pro Ala
- Asn Ile Thr Val Thr Ala Val Ala Arg Asn Pro
- Arg Trp Leu Ser Val Thr Trp Gln Asp Pro His
- Ser Trp Asn Ser Ser Phe Tyr Arg Leu Arg Phe
- Glu Leu Arg Tyr Arg Ala Glu Arg Ser Lys Thr
- Phe Thr Thr Trp Met Val Lys Asp Leu Gln His
- His Cys Val Ile His Asp Ala Trp Ser Gly Leu
- Arg His Val Val Gln Leu Arg Ala Gln Glu Glu
- Phe Gly Gln Gly Glu Trp Ser Glu Trp Ser Pro
- Glu Ala Met Gly Thr Pro Trp Thr Glu Ser Arg
- Ser Pro Pro Ala Glu Asn Glu Val Ser Thr Pro
- Met Gln Ala Leu Thr Thr Asn Lys Asp Asp Asp
- Asn Ile Leu Phe Arg Asp Ser Ala Asn Ala Thr
- Ser Leu Pro Val Gln Asp Ser Ser Ser Val Pro
- Leu Pro Thr Phe Leu Val Ala Gly Gly Ser Leu
- Ala Phe Gly Thr Leu Leu Cys Ile Ala Ile Val
- Leu Arg Phe Lys Lys Thr Trp Lys Leu Arg Ala
- Leu Lys Glu Gly Lys Thr Ser Met His Pro Pro
- Tyr Ser Leu Gly Gln Leu Val Pro Glu Arg Pro
- Arg Pro Thr Pro Val Leu Val Pro Leu Ile Ser
- Pro Pro Val Ser Pro Ser Ser Leu Gly Ser Asp
- Asn Thr Ser Ser His Asn Arg Pro Asp Ala Arg
- Asp Pro Arg Ser Pro Tyr Asp Ile Ser Asn Thr
- Asp Tyr Phe Phe Pro Arg,
- (C-terminal).
42 more elements
Dependencies are inferred from the claim text.