Nucleic acid encoding a family of acetyl-coenzyme-A transporter proteins, and products related thereto
AssigneeThe Burnham Institute
InventorsMinoru Fukuda, Akiko Kanamori, Yoshio Hirabayashi
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Abstract
In accordance with the present invention, there are provided isolated mammalian Acetyl-Coenzyme A Transporter (AT) proteins, anti-AT antibodies, therapeutic compositions, and nucleic acids encoding such. Bioassays and therapeutic methods employing invention AT proteins are also provided.
Claims19
An isolated nucleic acid encoding a mammalian acetyl-coenzyme A transporter (AT) protein, or a fragment thereof, wherein said nucleic acid is selected from:
- (a) DNA encoding the amino acid sequence set forth in SEQ ID NO:2, or
- (b) DNA that hybridizes to the DNA of (a) under moderately stringent conditions, wherein said DNA encodes biologically active AT, or
- (c) DNA degenerate with respect to either (a) or (b) above, wherein said DNA encodes biologically active AT, or
- (d) splice variant cDNA sequences thereof.
An oligonucleotide comprising at least 14 nucleotides capable of specifically hybridizing with a sequence of nucleic acids of the nucleotide sequence set forth in SEQ ID NO:1.
A single strand DNA primer for amplification of AT nucleic acid, wherein said primer is selected from a nucleic acid sequence derived from the nucleic acid sequence set forth as SEQ ID NO:
- 1.
A bioassay for evaluating whether test compounds are capable of acting as agonists or antagonists for acetyl-coenzyme A transporter (AT) proteins characterized by being able to transport acetyl-coenzyme A (Ac-CoA) across a membrane, or functional modified forms of said AT proteins, said bioassay comprising:
- (a) culturing cells containing:
- DNA which expresses AT protein(s) or functional modified forms thereof,
- wherein said culturing is carried out in the presence of at least one compound whose ability to modulate Ac-CoA transport activity of AT protein is sought to be determined, and thereafter
- (b) monitoring said cells for either an increase or decrease in the level of Ac-CoA.
A bioassay for evaluating whether test compounds are capable of acting as antagonists for AT protein(s) characterized by being able to transport acetyl-coenzyme A across a membrane, or functional modified forms of said AT protein(s), said bioassay comprising:
- (a) culturing cells containing:
- DNA which expresses AT protein(s), or functional modified forms thereof,
- wherein said culturing is carried out in the presence of:
- increasing concentrations of at least one compound whose ability to inhibit Ac-CoA transport activity of AT protein(s) is sought to be determined, and
- a fixed concentration of Ac-CoA;
- and thereafter
- (b) monitoring in said cells the level of Ac-CoA transported into intracellular organelles as a function of the concentration of said compound, thereby indicating the ability of said compound to inhibit AT transport activity.
A bioassay for evaluating whether test compounds are capable of acting as antagonists for AT protein(s) characterized by being able to transport acetyl-coenzyme A across a membrane, or functional modified forms of said AT protein(s), said bioassay comprising:
- (a) culturing cells containing:
- DNA which expresses AT protein(s), or functional modified forms thereof,
- wherein said culturing is carried out in the presence of:
- fixed concentrations of at least one compound whose ability to inhibit Ac-CoA transport activity of AT protein(s) is sought to be determined, and
- an increasing concentration of Ac-CoA;
- and thereafter
- (b) monitoring in said cells the level of Ac-CoA transported into intracellular organelles as a function of the concentration of said compound, thereby indicating the ability of said compound to inhibit AT transport activity.
A bioassay for evaluating whether test compounds are capable of acting as agonists for AT protein(s) characterized by being able to transport acetyl-coenzyme A across a membrane, or functional modified forms of said AT protein(s), said bioassay comprising:
- (a) culturing cells containing:
- DNA which expresses AT protein(s), or functional modified forms thereof,
- wherein said culturing is carried out in the presence of:
- increasing concentrations of at least one compound whose ability to increase Ac-CoA transport activity of AT protein(s) is sought to be determined, and
- a fixed concentration of Ac-CoA;
- and thereafter
- (b) monitoring in said cells the level of Ac-CoA transported into intracellular organelles as a function of the concentration of said compound, thereby indicating the ability of said compound to increase AT transport activity.
A bioassay for evaluating whether test compounds are capable of acting as agonists for AT protein(s) characterized by being able to transport acetyl-coenzyme A across a membrane, or functional modified forms of said AT protein(s), said bioassay comprising:
- (a) culturing cells containing:
- DNA which expresses AT protein(s), or functional modified forms thereof,
- wherein said culturing is carried out in the presence of:
- fixed concentrations of at least one compound whose ability to increase Ac-CoA transport activity of AT protein(s) is sought to be determined, and
- an increasing concentration of Ac-CoA;
- and thereafter
- (b) monitoring in said cells the level of Ac-CoA transported into intracellular organelles as a function of the concentration of said compound, thereby indicating the ability of said compound to increase AT transport activity.
Dependencies are inferred from the claim text.