US 5,851,780 AGranted

Genomic DNA of human cholesterol 7.alpha.-hydroxylase and methods for using it

AssigneeNortheastern Ohio University

InventorJohn Young Ling Chiang

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Abstract

Genomic DNA of cholesterol 7.alpha.-hydroxylase and a minigene are disclosed. The minigene is used for making a transgenic animal that produces functionally active cholesterol 7.alpha.-hydroxylase and functions as a disease model. A cholesterol 7.alpha.-hydroxylase promoter region and reporter gene construct is provided, as well as a transgenic animal that expresses the promoter/reporter gene.

Claims3

Dependent claims

A method for screening a compound for its effect on non-truncated human CYP7 enzyme activity, comprising the steps of:

(a) contacting catalytically active, truncated human CYP7 according to claim 1 with a compound;
and
(b) measuring the catalytic activity of the catalytically active, truncated human CYP7.

Dependencies are inferred from the claim text.

Facts

Application: 08/477,952Filed 7 Jun 1995 · continuation
Priority: 13 Oct 1993
Granted: 22 Dec 1998
Term ends: Not estimatedThe source records don’t support an estimate.

Classification30 codes

G01N 33/6872
Involving proteins, peptides or amino acids: intracellular protein regulatory factors and their receptors, e.g. including ion channels
A01K 67/0275
New or modified breeds of vertebrates: genetically modified vertebrates, e.g. transgenic
A01K 67/0278
Genetically modified vertebrates, e.g. transgenic: knock-in vertebrates, e.g. humanised vertebrates
C07K 14/47
From vertebrates: from mammals

Family

Serial135,488Parent of this continuation · filed 13 Oct 1993
Serial361,458Parent of this continuation · filed 21 Dec 1994
US 5,851,780 AThis patent

From the US filing history. Foreign family members aren’t listed.

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