Loading patent…
AssigneeCogent Limited
InventorsRuth Anne McAdam, Jeremy Watson Dale, Zainul Fadziruddin Bin Zainuddin, David Catty
Estimating the term…
The invention provides nucleotide probes, kits and methods for the detection and differentiation of Mycobacteria. The gene probes, kits and methods are useful for the diagnosis of tuberculosis and/or for epidemiological study tools for investigating the progress of infections caused by Mycobacteria. The gene probes as provided comprise part or all of nucleotide sequences provided in the soecification or an allele or a derivative of the nucleotide sequences. The gene probes can distinguish between M.tuberculosis, M.bovis and BCG as well as being able to distinguish between different strains of M.tuberculosis. The probes do not show significant hybridisation to nucleic acids from M.paratuberculosis, M.intracellulare, M.scrofulaceum, M.phlei, M.fortuitum, M.kansasii, M.avium, M.malnioense. M.flavescens, M.gordonae and M.chelonei.
A nucleotide probe for the diagnosis and/or epidemiological study of Mycobacterial infection that specifically hybridizes to nucleic acid from members of the Mycobacterium tuberculosis complex but not to nucleic acid from mycobacteria that are not members of the complex, wherein said nucleotide probe comprises, or hybridizes with, Mycobacterium tuberculosis DNA from recombinant phage EMBL4/A-3 (accession number NCTC 12380).
A nucleotide probe for the diagnosis and/or epidemiological study of Mycobacterial infection that specifically hybridizes to nucleic acid from members of the M. tuberculosis complex but not to nucleic acid from mycobacteria that are not members of the complex, wherein said nucleotide probe comprises, or specifically hybridizes under stringent conditions with an approximately 1.9 kb nucleotide sequence which sequence, in the genome of Mycobacterium tuberculosis strain 50410, occurs downstream of the 3' end of the nucleotide sequence shown in FIG. 2 (SEQ ID NO:1) of the drawings, as depicted as fragment 5A in FIG. 1 of the drawings, or its complementary strand thereof.
Dependencies are inferred from the claim text.