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US 5,851,554 AGranted
Purified cardiac troponin I
AssigneeSpectral Diagnostics, Inc.
InventorsLillian Lee, George Jackowski
Life of this patent
Estimating the term…
Abstract
A method is provided for isolating substantially intact cardiac troponin I from cardiac tissue comprising extracting the, troponin I and purifying it in the presence of an effective amount of a mixture of protease inhibitors. The human cardiac troponin I prepared by the present method is characterised by a molecular weight of about 28 kDa.
Claims13
A pure, stable cardiac troponin I preparation, the troponin I having a molecular weight of about 28 kDa, prepared by a process which comprises the steps of:
- extracting human cardiac tissue with an aqueous extraction buffer at a pH of from about 7 to 9 which is 8 to 10M in Urea and contains a protease inhibitor mixture comprising at least two cathepsin protease inhibitors, at least one serine protease inhibitor and at least one cysteine protease inhibitor;
- separating the troponin I from the extract by affinity chromatography in the presence of the extraction buffer and calcium ion thereby to absorb the troponin I;
- and desorbing the troponin I by washing with the aqueous extraction buffer containing EGTA.
Dependencies are inferred from the claim text.