Materials and method for the modification of plant lignin content
AssigneesGenesis Research & Development Corporation Limited, Fletcher Challenge Forests Limited
InventorsLeonard N. Bloksberg, Ilkka Havukkala, Alastair Grierson
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Abstract
Novel isolated DNA sequences associated with the lignin biosynthetic pathway are provided, together with DNA constructs including such sequences. Methods for the modulation of lignin content in plants are also disclosed, the methods comprising incorporating one or more of the inventive DNA sequences or a sequence complementary to an inventive DNA sequence into the genome of a plant.
Claims39
An isolated DNA sequence comprising a nucleotide sequence selected from the group consisting of:
- (a) sequences recited in SEQ ID NO:
- 1-6 and 8-13;
- (b) complements of the sequences recited in SEQ ID NO:
- 1-6 and 8-13;
- (c) reverse complements of the sequences recited in SEQ ID NO:
- 1-6 and 8-13;
- (d) reverse sequences of the sequences recited in SEQ ID NO:
- 1-6 and 8-13;
- and
- (e) sequences having at least about a 99% probability of being the same as a sequence of (a)-(d) as measured by computer algorithm FASTA.
A DNA construct comprising, in the 5'-3' direction:
- (a) a gene promoter sequence;
- (b) at least one open reading frame coding for at least one functional portion of an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 2-6, 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 2-6, 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
A DNA construct comprising, in the 5' to 3' direction:
- (a) a gene promoter sequence;
- (b) at least one non-coding region of a gene coding for an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 1-6, 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 1-6, 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
A transgenic plant cell comprising a DNA construct, the DNA construct comprising, in the 5'-3' direction:
- (a) a gene promoter sequence;
- (b) at least one open reading frame coding for at least one functional portion of an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 2-6, 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 2-6 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
A transgenic plant cell comprising a DNA construct, the DNA construct comprising, in the 5'-3' direction:
- (a) a gene promoter sequence;
- (b) at least one non-coding region of a gene coding for an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 1-6, 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 1-6, 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
A method for modulating the lignin content of a plant comprising stably incorporating into the genome of the plant a DNA construct comprising, in the 5'-3' direction:
- (a) a gene promoter sequence;
- (b) at least one open reading frame coding for at least one functional portion of an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 2-6, 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 2-6, 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
A method for producing a plant having altered lignin structure comprising:
- (a) transforming a plant cell with a DNA construct comprising, in the 5'-3' direction, a gene promoter sequence, at least one open reading frame coding for at least one functional portion of an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 2-6 and 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 2-6, 8-13 as measured by computer algorithm FASTA, and a gene termination sequence lo provide a transgenic cell;
- (b) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
A method of modifying the activity of an enzyme in a plant comprising stably incorporating into the genome of the plant a DNA construct including
- (a) a gene promoter sequence;
- (b) at least one open reading frame coding for at least one functional portion of an enzyme encoded by a nucleotide sequence selected from the group consisting of sequences recited in SEQ ID NO:
- 2-6 and 8-13 and sequences having at least about a 99% probability of being the same as a sequence recited in SEQ ID NO:
- 2-6, 8-13 as measured by computer algorithm FASTA;
- and
- (c) a gene termination sequence.
Dependencies are inferred from the claim text.