Alteration of amino acid compositions in seeds
AssigneePioneer Hi-Bred International, Inc.
InventorsRudolf Jung, Craig Hastings, Sean Coughlan, David Hu
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Abstract
The present invention provides methods for increasing the level of preselected amino acids in seeds of plants, thereby enhancing the nutritional value of the seeds, by genetic modification. The present invention is particularly useful in increasing the methionine, lysine, and/or cysteine content in seeds of plants. Also provided, are isolated endogenous DNA molecules which encode soybean albumins. The present invention also provides an antibody which is capable of specifically binding to soybean albumins. The present invention further provides methods for isolating and purifying 2S albumins.
Claims28
An isolated DNA molecule comprising a preselected DNA segment which encodes a protein having SEQ ID NOS:
- 2, 4 or 6.
An isolated DNA molecule comprising a preselected DNA segment comprising the sequence of SEQ ID NOS:
- 1, 3 or 5.
A DNA molecule wherein SEQ ID NOS:
- 1 or 3 are modified by site-directed mutagenesis to encode for a protein with increased levels of lysine, methionine, cysteine or combinations thereof.
A DNA molecule wherein SEQ ID NOS:
- 1 or 3 are modified by site-directed mutagenesis to encode for a protein with decreased levels of asparagine and/or glutamine.
Dependencies are inferred from the claim text.