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AssigneeBio Merieux
InventorsClaude Mabilat, Jean-Claude Pechere
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Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinum.
A hybridization probe consisting of a nucleotide sequence that is at least 85% homologous with SEQ ID NO:
A species probe capable of specifically hybridizing with a single-stranded fragment of a species of the genus Mycobacterium and not with nucleic acid of other species of the genus Mycobacterium, said fragment consisting of a nucleotide sequence consisting of nucleotides 438 to 751 of a gene coding for a 65-kD mycobacterial antigen of said species, or its complementary sequence.
A specific primer for the amplification of genomic DNA of Mycobacterium, said primer consisting of from 5 to 30 monomers and being capable of specifically hybridizing with a single-stranded mycobacterial fragment of at least nineteen species of the genus Mycobacterium, each said mycobacterial fragment consisting of a nucleotide sequence consisting of nucleotides 438 to 751 of a gene coding for a 65-kD mycobacterial antigen of each said species, or its complementary sequence.
A specific primer for the amplification of genomic DNA of a species of Mycobacterium, said primer being capable of specifically hybridizing with a single-stranded fragment of a species of the genus Mycobacterium and not with nucleic acid of other species of the genus Mycobacterium, said fragment consisting of a nucleotide sequence consisting of nucleotides 438 to 751 of a gene coding for a 65-kD mycobacterial antigen of said species, or its complementary sequence.
A genus probe capable of specifically hybridizing with a single-stranded mycobacterial fragment of at least nineteen species of the genus Mycobacterium, each said mycobacterial fragment consisting of a nucleotide sequence consisting of nucleotides 438 to 751 of a gene coding for a 65-kD mycobacterial antigen of each said species, or its complementary sequence, wherein said genus probe consists of 5 to 100 monomers.
A method for selectively detecting Mycobacterium in a biological sample, said method comprising:
Dependencies are inferred from the claim text.