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AssigneeNational Science Council
InventorsNa-Sheng Lin, Yau-Heiu Hsu
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A satellite RNA was found to be naturally associated with bamboo mosaic virus (BaMV-V) isolated from infected Bambusa vulgaris McClure. Nucleotide sequence revealed that this satellite RNA genome contains 836 nucleotides and encodes a 20 kDa protein. Infectious transcripts have been generated from full length cDNA downstream T7 RNA polymerase promoter. Precise replacement of open reading frame (ORF) of cDNA of satellite RNA with sequence encoding bacterial CAT (chloramphenicol acetyltransferase) resulted in high level expression of CAT in infected dicotyledon plants, Chenopodium quinoa and tobacco (Nicotiana benthamiana) in the presence of baMV genomic RNA. Thus, this satellite system is potentially useful as a satellite-based plant expression vector.
A purified and isolated viral satellite RNA for use as a vector for plant foreign gene expression which consists of the sequence shown in FIG. 3 (SEQ ID:1).
A method of constructing a plasmid comprising an infectious cDNA clone which produces infectious viral satellite RNA virions for plant infection in the presence of a BaMV genomic RNA, comprising:
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