Extraction of myelin basic protein
AssigneeOy Aboatech AB
InventorsJorma Maatta, Ari Hinkkanen
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Abstract
A process for extraction of myelin basic protein from myelin containing tissue, such as central nervous system tissue, which process comprises the following steps: extraction of the myelin basic protein from myelin containing tissue with an organic solvent selected from the group consisting of chloroform and compounds having a polarity similar to that of chloroform; incubation of the organic phase in the presence of a lower aliphatic alcohol or propylene glycol; transfer of the myelin basic protein from the lower aliphatic alcohol/organic solvent mixture to an aqueous phase with the aid of hydrogen ions (protons); and recovery of the purified myelin basic protein. The invention also relates to the product obtainable by the process.
Claims12
Process for extracting myelin basic protein from myelin containing tissue, which process consists essentially of the following steps:
- extracting the myelin basic protein from the myelin containing tissue with an organic solvent selected from the group consisting of chloroform and compounds having a polarity similar to that of chloroform to produce an organic phase containing myelin basic protein;
- incubating the organic phase in a mixture with a lower aliphatic alcohol or propylene glycol;
- transferring the myelin basic protein from the mixture of the lower aliphatic alcohol or propylene glycol and organic solvent, to an aqueous phase containing hydrogen ions (protons);
- and
- recovering the purified myelin basic protein.
Dependencies are inferred from the claim text.