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OwnerOrla M. Conneely, Denis R. Headon +2inventors
InventorsOrla M. Conneely, Denis R. Headon, Bert W. O'Malley, Gregory S. May
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The verified cDNA sequences for human, bovine and porcine lactoferrin protein have been used to prepare recombinant lactoferrin for therapeutic and nutritional applications. Regions of the cDNA such as the Fe binding sites can be used to make an hLF polypeptide product. The present invention provides novel plasmids, transfected eucaryotic cells and methods of producing these plasmids and transfected eucaryotic cells. The novel plasmid contains the cDNA for lactoferrin protein. Methods for the production of lactoferrin protein in fungi and bacteria are also provided. Thus, the present invention provides an efficient and economical means for the production of recombinant lactoferrin protein and lactoferrin related polypeptides.
An isolated lactoferrin product produced by the expression of a nucleotide segment having the coding sequence of a naturally-occurring lactoferrin allele in a fungal host cell transformed with said lactoferrin-encoding nucleotide sequence, wherein said lactoferrin-encoding nucleotide sequence is a cDNA and said cDNA is operably linked to a promoter element utilized by said fungal host cell.
An isolated lactoferrin product produced by the expression of a nucleotide segment having the coding sequence of a naturally-occurring lactoferrin allele in a fungal host cell transformed with said lactoferrin-encoding nucleotide sequence, wherein said lactoferrin-encoding nucleotide sequence is a cDNA and said cDNA is operably linked to a promoter element utilized by said fungal host cell, wherein said lactoferrin is deglycosylated after expression.
An isolated lactoferrin fragment produced by the expression of a nucleotide segment encoding a polypeptide having the amino acid sequence of SEQ ID NO:2 from position 20 through position 71, inclusive, in a fungal host cell transformed with said lactoferrin fragment-encoding nucleotide sequence, wherein said lactoferrin fragment-encoding nucleotide sequence is a cDNA sequence and said cDNA sequence is operably linked to a promoter element utilized by said fungal host cell.
An isolated lactoferrin iron-binding lobe produced by the expression of a nucleotide sequence encoding a lactoferrin iron-binding lobe comprising a single iron-binding site of a naturally-occurring lactoferrin allele in a fungal host cell transformed with said lactoferrin iron-binding lobe-encoding nucleotide sequence, wherein said nucleotide coding nucleotide sequence is a cDNA sequence and said cDNA sequence is operably linked to a promoter element utilized by said fungal host cell.
A human lactoferrin protein manufactured by a recombinant process of production comprising the following steps:
A deglycosylated lactoferrin protein manufactured by a recombinant process of production comprising the following steps:
A human lactoferrin protein fragment manufactured by a recombinant process of production comprising the following steps:
A lactoferrin protein manufactured by a recombinant process comprising the following steps:
Dependencies are inferred from the claim text.