Bone morphogenetic protein (BMP)--6
AssigneeGenetics Institute, Inc.
InventorsJohn M. Wozney, Elizabeth A. Wang, Vicki A. Rosen, Anthony J. Celeste
Life of this patent
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Abstract
Purified bone morphogenetic protein (BMP)-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Claims15
A purified human bone morphogenetic protein-6 produced by the steps of
- (a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the DNA sequence of FIG. 3 from nucleotide 1321 to 1698;
- and
- (b) recovering from said culture medium a protein comprising the amino acid sequence from amino acid 388 to amino acid 513 as shown in FIG. 3.
A composition comprising a purified BMP-6! bone morphogenetic protein-6 produced by the steps of
- (a) transforming a host cell with a DNA sequence comprising nucleotides #160 to #1712 of FIG. 3;
- (b) culturing said transformed cell in a suitable culture medium;
- and
- (c) isolating and purifying said protein from said culture medium.
A purified bone morphogenetic protein-6 (BMP-6) comprising an amino acid sequence selected from the group consisting of:
- (a) amino acids 412 through 513 of FIG. 3;
- (b) amino acids 388 through 513 of FIG. 3;
- and
- (c) amino acids 382 through 513 of FIG. 3.
A BMP-6 protein encoded by a DNA sequence comprising a DNA sequence selected from the group consisting of the DNA molecules of ATCC deposits 68245 and 68021.
A BMP-6 protein comprising amino acids 382 through 513 of FIG. 3.
A purified BMP-6 polypeptide comprising an amino acid sequence encoded by a DNA sequence comprising a DNA sequence selected from the group consisting of:
- (a) nucleotides 1393 through 1698 of FIG. 3;
- (b) nucleotides 1321 through 1698 of FIG. 3;
- (c) nucleotides 1303 through 1698 of FIG. 3;
- and
- (d) naturally occurring allelic sequences and degenerative codon sequences of (a) through (c).
A purified BMP-6 polypeptide encoded by a DNA sequence which hybridizes under stringent wash conditions of 0.1.times. SSC at 65.degree. C. to a DNA sequence selected from the group consisting of:
- (a) nucleotides 1393 through 1698 of FIG. 3;
- (b) nucleotides 1321 through 1698 of FIG. 3;
- (c) nucleotides 1303 through 1698 of FIG. 3;
- and
- (d) naturally occurring allelic sequences and degenerative codon sequences of (a) through (c).
A purified BMP-6 polypeptide comprising an amino acid sequence selected from the group consisting of amino acid sequences encoded by a DNA sequence comprising nucleotides 289 through 666 of FIG. 2.
Dependencies are inferred from the claim text.