Acid-labile subunit als of insulin-like growth factor binding proteincomplex
AssigneeGenetech, Inc.
InventorRobert Charles Baxter
Life of this patent
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Abstract
An acid-labile protein is described that, when incubated with the 53-Kd acid-stable protein occupied by IGF, converts it to a high molecular weight complex, corresponding to the in vivo form of IGF. This protein is called the acid-labile subunit (ALS) of IGF binding protein complex and is provided in biologically pure form. ALS is useful in treating wounds and promoting cellular growth. The nucleic acid encoding ALS, antibodies binding thereto, and fragments thereof are also disclosed.
Claims10
A method for promoting cellular growth in animals or humans comprising administering to said animal or human a therapeutically effective amount of a composition comprising the acid-labile sub-unit (ALS) of insulin-like growth factor binding protein complex, wherein in vivo, the ALS is in complexed form with insulin-like growth factor-I and acid-stable insulin-like growth factor binding protein, said ALS being purified to the extent necessary to obtain the following partial N-terminal amino acid sequence:
- Gly
- AspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAlaCys Ala
- wherein the first amino acid may be Gly or Ala (Seq. ID Nos. 1 and 2, respectively, with Seq. ID No. 1 starting with Gly and Seq. ID No. 2 starting with Ala), and characterized by its inability to bind to uncomplexed insulin-like growth factor-I, insulin-like growth factor-II, or acid stable insulin-like growth factor binding protein and its ability to bind to the acid-stable insulin-like growth factor binding protein when complexed with insulin-like growth factor-I.
A method for promoting cellular growth in animals or humans comprising administering to said animal or human a therapeutically effective amount of a composition comprising the acid-labile sub-unit (ALS) of insulin-like growth factor binding protein complex and a pharmaceutically-acceptable carrier, wherein in vivo, the ALS is in complexed form with insulin-like growth factor-I and acid-stable insulin-like growth factor binding protein, said ALS being purified to the extent necessary to obtain the following partial N-terminal amino acid sequence:
- Gly
- AspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAlaCys Ala
- wherein the first amino acid may be Gly or Ala (Seq. ID Nos. 1 and 2, respectively, with Seq. ID No. 1 starting with Gly and Seq. ID No. 2 starting with Ala), and characterized by its inability to bind to uncomplexed insulin-like growth factor-I, insulin-like growth factor-II, or acid stable insulin-like growth factor binding protein and its ability to bind to the acid-stable insulin-like growth factor binding protein when complexed with insulin-like growth factor-I.
Dependencies are inferred from the claim text.