Method for monitoring the effects of chemotherapeutic agents on neoplasmic media
OwnerRobert R. Alfano, Guichen C. Tang +1inventors
InventorsRobert R. Alfano, Guichen C. Tang, Stimson P. Schantz
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Abstract
A method for monitoring the effects of a chemotherapeutic agent on a neoplasm medium. The method is premised on the discovery that chemotherapeutic agent, such as retinoic acid, affects the fluorescence spectroscopy of neoplasmic media and that such differences can be monitored, for example, by comparing the spectral profiles, spectral peaks, and spectral bandwidths of fluorescence at various wavelengths of the medium before and after administration of the chemotherapeutic agent. Differences in the excitation spectroscopy of the medium can also be used to monitor the effects of the agent.
Claims18
A method for monitoring the effect of retinoic acid on a neoplasmic medium, the method comprising the steps of:
- a) prior to administering retinoic acid to the neoplasmic medium, illuminating the neoplasmic medium with a beam of substantially monochromatic light;
- b) then, detecting fluorescent light emitted from the neoplasmic medium at one or more wavelengths;
- c) then, administering retinoic acid to the neoplasmic medium;
- d) then, illuminating the neoplasmic medium with said beam of substantially monochromatic light;
- e) then, detecting fluorescent light emitted from the neoplasmic medium at said one or more wavelengths;
- f) then, comparing the intensity of fluorescent light detected from the neoplasmic medium before and after administration of retinoic acid.
A method for monitoring the effect of retinoic acid on a neoplasmic medium, the method comprising the steps of:
- a) prior to administering retinoic acid to the neoplasmic medium, illuminating the neoplasmic medium with a beam of substantially monochromatic light having a changeable wavelength over a spectrum of wavelengths;
- b) then, detecting fluorescent light emitted from the neoplasmic medium at a predetermined emission wavelength;
- c) then, administering retinoic acid to the neoplasmic medium;
- d) then, illuminating the neoplasmic medium with said beam of substantially monochromatic light having a changeable wavelength over said spectrum of wavelengths;
- e) then, detecting fluorescent light emitted from the neoplasmic medium at said predetermined emission wavelength;
- and
- f) then, comparing the intensity of fluorescent light detected from the neoplasmic medium before and after administration of retinoic acid.
A method for monitoring the effect of retinoic acid on a neoplasmic medium, the method comprising the steps of:
- (a) prior to administering retinoic acid to the neoplasmic medium, illuminating the neoplasmic medium with a beam of substantially monochromatic light, said beam of substantially monochromatic light having a wavelength of approximately 340 nm;
- (b) then, detecting fluorescent light emitted from the neoplasmic medium at one or more wavelengths between about 360 nm and 660 nm;
- (c) then, administering retinoic acid to the neoplasmic medium;
- (d) then, illuminating the neoplasmic medium with said beam of substantially monochromatic light;
- (e) then, detecting fluorescent light emitted from the neoplasmic medium at said one or more wavelengths between about 360 nm and 660 nm;
- (f) then, comparing the intensity of fluorescent light detected from the neoplasmic medium before and after administration of retinoic acid.
Dependencies are inferred from the claim text.