Culturing monocytes with IL-4, TNF-.alpha. and GM-CSF TO induce differentiation to dendric cells
AssigneeDuke University
InventorsThomas F. Tedder, Liang-Ji Zhou
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Abstract
Disclosed is a method for inducing the differentiation of a monocyte into a dendritic cell, the method including the culturing of a monocyte in a medium including GM-CSF, IL-4, and TNF-.alpha..
Claims22
A method for inducing the differentiation of a population of monocytes into a population of cells comprising greater than 50% mature CD83.sup.+ dendritic cells, said method comprising culturing said monocytes in an induction medium comprising granulocyte/macrophage-colony stimulating factor ("GM-CSF"), interleukin-4 ("IL-4"), and tumor necrosis factor-.alpha. ("TNF-.alpha."), said GM-CSF, IL-4, and TNF-.alpha. being present simultaneously in said induction medium in sufficient amounts to induce said differentiation, said culturing resulting in said induction of differentiation.
A method for generating a population of greater than 50% mature CD83.sup.+ dendritic cells, said method comprising:
- (a) inducing the differentiation of a population of monocytes into said population of dendritic cells, by culturing said population of monocytes in an induction medium comprising granulocyte/macrophage-colony stimulating factor ("GM-CSF"), interleukin-4 ("IL-4"), and tumor necrosis factor-.alpha. ("TNF-.alpha."), said GM-CSF, IL-4, and TNF-.alpha. being simultaneously present in said induction medium in sufficient amounts to induce said differentiation;
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- (b) recovering said population of said dendritic cells.
Dependencies are inferred from the claim text.