Cloning and expression of host-protective immunogens of IBDV
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsAhmed Abdullah Azad, Peter John Hudson, Kevin John Fahey
Life of this patent
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Abstract
A recombinant DNA molecule comprising a nucleotide sequence substantially corresponding to all or a portion of IBDV RNA, particularly the IBDV RNA segment of approximately 3400 b.p. In particular, a DNA molecule coding for all or a part of at least one structural protein of IBDV, for example, the 32 Kd and/or the 41/37 Kd structural proteins. Synthetic peptides or polypeptides, and fused polypeptides, prepared by expression of host cells containing these DNA molecules are also disclosed.
Claims14
An isolated recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide which includes the conformational epitope of the 41/37 Kd protein of IBDV serotype I that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is capable of eliciting IBDV neutralizing antibodies in an avian host.
An isolated A recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide which includes the conformational epitope encoded by the Ndel-Nael fragment of the IBDV genome sequence coding for the 41/37 KD protein of IBDV serotype I and is capable of eliciting IBDV neutralizing antibodies in an avian host.
An isolated recombinant DNA molecule comprising a nucleotide sequence substantially as shown in FIGS. 10A-10F, or a portion of said base sequence which codes for a polypeptide which includes the confirmational epitope of the 41/37 Kd protein of IBDV serotype I that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is capable of eliciting IBDV neutralizing antibodies in an avian host.
An isolated recombinant DNA molecule comprising a nucleotide sequence which codes for a serotype I IBDV 41/37 Kd polypeptide which retains a conformational neutralizing epitope of the native, intact IBDV virus that is recognized by IBDV neutralizing monoclonal antibody MAb 17-82 and is are absent from SDS-detergent-treated 41/37 Kd polypeptide.
Dependencies are inferred from the claim text.