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US 5,849,574 AGranted
Human tPA production using vectors coding for DHFR protein
AssigneeGenentech, Inc.
InventorsArthur D. Levinson, Diane Pennica, William J. Kohr, Gordon A. Vehar, David V. Goeddel, Elizabeth M. Yelverton, Christian C. Simonsen
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Abstract
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
Claims1
An expression vector which comprises:
- a first DNA sequence encoding a DHFR protein;
- and
- a second DNA sequence encoding human tPA;
- wherein each of said first and second sequences is operably linked to a DNA sequence capable of effecting its expression in a CHO cell line transformed with said vector.
1 more element
Dependencies are inferred from the claim text.