Method for the production of non-group C adenoviral vectors
AssigneeCornell Research Foundation, Inc.
InventorErik S. Falck-Pedersen
Life of this patent
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Abstract
A method of producing a replication deficient adenovirus comprising a passenger gene and a deficiency in an essential gene function of the E1 region of an adenovirus comprising producing the adenovirus in a cell that provides in trans gene functions of the E1 and E4 regions of one or more adenoviruses not belonging to the same serogroup as the replication deficient adenovirus.
Claims14
A method of producing a replication deficient adenovirus comprising a passenger gene, said replication deficient adenovirus having a first serotype belonging to a first serogroup and having a genome deficient in an essential gene function of the E1 region of the adenoviral genome, said method comprising
- (a) transferring DNA comprising said adenoviral genome to a cell that provides in trans
- (i) one or more essential gene functions of the E1 region of an adenoviral genome derived from an adenoviral genome having a second serotype belonging to a second serogroup, and
- (ii) one or more gene functions of the E4 region of an adenoviral genome derived from an adenoviral genome having a third serotype belonging to said second serogroup,
- wherein the first serogroup is different than the second serogroup,
- (b) maintaining said cell to produce said replication deficient adenovirus.
Dependencies are inferred from the claim text.