Arabinoxylan degrading enzymes
AssigneeGist-Brocades N.V.
InventorsMonique Josina Andrea Van Der Wouw, Albert Johannes Joseph Van Ooijen, Marcus Matheus Catharina Gielkens, Leendert Hendrik De Graaff, Jacob Visser
Life of this patent
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Abstract
Methods and expression constructs are provided for the cloning and overexpression of an arabinoxylan degrading enzyme of fungal origin in a selected microbial host cell. The enzyme is shown to be active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.
Claims21
Recombinant DNA comprising a nucleotide sequence encoding a polypeptide having arabinoxylan degrading activity, or a polypeptide precursor thereof, wherein said nucleotide sequence is selected from:
- (a) a nucleotide sequence encoding a polypeptide having the amino acid sequence represented by amino acids 1 to 306, or a polypeptide precursor of said polypeptide represented by amino acids -27 to 306 in SEQ ID NO:
- 6;
- (b) a nucleotide sequence encoding a polypeptide having the amino acid sequence represented by amino acid 1 to 306, or a precursor of said polypeptide represented by amino acids -27 to 306 in SEQ ID NO:
- 8;
- (c) a nucleotide sequence encoding a portion of the polypeptides represented by amino acid residues 1 to 306 depicted in SEQ ID NO;
- 6 or 8, wherein said portion retains arabinoxylan degrading activity;
- (d) a nucleotide sequence encoding a polypeptide having arabinoxylan degrading activity and having the nucleotide sequence represented by nucleotides 784 to 1779 in SEQ ID NO:
- 5 or nucleotides 823 to 1818 in SEQ ID NO:
- 7;
- (e) a nucleotide sequence encoding a polypeptide having arabinoxylan degrading activity, or portion thereof wherein said portion has arabinoxylan degrading activity, which polypeptide or portion is encoded by a nucleotide sequence which hybridizes to a DNA fragment as represented by nucleotides 784 to 1779 in SEQ ID NO:
- 5 or nucleotides 823 to 1818 in SEQ ID NO:
- 7 or the complements thereof under hybridization conditions which include a first wash at 65.degree. C. for 30 minutes in 5.times.SSC/0.1% SDS followed by a second wash at 65.degree. C. for 30 minutes in 2.times.SSC/0.1% SDS followed by a third wash at 65.degree. C. for 30 minutes in 0.1.times.SSC/0.1% SDS followed by a fourth wash at 65.degree. C. for 30 minutes in 0.1.times.SSC.
A recombinant DNA comprising a DNA represented by nucleotides 1 to 783 of SEQ ID NO:
- 5, or a fragment thereof, capable of regulating expression of a DNA sequence attached to it.
A recombinant DNA comprising a DNA represented by nucleotides 1 to 822 of SEQ ID NO. 7, or a fragment thereof, capable of regulating expression of a DNA sequence attached to it.
The recombinant DNA of claim I which comprises a nucleotide sequence encoding a polypeptide having the amino acid sequence represented by amino acids 1 to 306, or a polypeptide precursor of said polypeptide represented by amino acids -27 to 306 in SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.