Discovery of and method for cloning and producing the PspGI restriction endonuclease
AssigneeNew England Biolabs, Inc.
InventorsRichard D. Morgan, Zhiyuh Chang
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Abstract
The present invention relates to the type II restriction endonuclease PspGI, obtainable from Pyrococcus species G-I-H (NEB #906), an enzyme which recognizes the DNA sequence 5' CC(A/T)GG 3' and cleaves before the first C of the recognition sequence to produce a 5 base 5' extension: 5'-.dwnarw.CC(A/T)GG-3' 3'-GG(T/A)CC.uparw.-5' (wherein G represents guanine, C represents cytosine, A represents adenine and T represents thymine and (A/T) represents either A or T in that one position.)
Claims8
Isolated DNA coding for the PspGI restriction endonuclease, wherein the isolated DNA is obtainable from Pyrococcus species G-I-H.
A recombinant DNA vector comprising a vector into which a DNA segment coding for the PspGI restriction endonuclease has been inserted.
Isolated DNA coding for the PspGI restriction endonuclease and methylase, wherein the isolated DNA is obtainable from ATCC No. 98435.
A substantially pure Type II restriction endonuclease endogenous to Pyrococcus species G-I-H recognizing the following base sequence in double-stranded deoxyribonucleic acid molecules:
- 5'-.dwnarw.CC(A/T)GG-3'
- 3'-GG(T/A)CC.uparw.-5'
- and having a cleavage position defined by the arrows.
Dependencies are inferred from the claim text.