Valyl trna polynucleotides from Streptococcus pneumoniae
AssigneeSmithKline Beecham Corporation
InventorsJames R. Brown, Deborah D. Jaworski, Elizabeth J. Lawlor, Min Wang
Life of this patent
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Abstract
The invention provides valS polypeptides and DNA (RNA) encoding valS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing valS polypeptides to screen for antibacterial compounds.
Claims27
An isolated polynucleotide comprising a polynucleotide encoding a polypeptide having at least a 95% identity to a polypeptide comprising amino acids 1 to 883 of SEQ ID NO:2.
An isolated polynucleotide comprising a polynucleotide having at least a 95% identity to a polynucleotide encoding the same mature valyl tRNA synthetase in deposit number NCIMB 40794 or NCIMB 40800.
An isolated polynucleotide comprising a polynucleotide encoding a polypeptide having at least a 97% identity to a polypeptide comprising amino acids 1 to 883 of SEQ ID NO:2.
An isolated polynucleotide comprising a polynucleotide having at least a 97% identity to a polynucleotide encoding the same mature valyl tRNA synthetase in deposit number NCIMB 40794 or NCIMB 40800.
An isolated polynucleotide comprising a polynucleotide encoding a polypeptide comprising amino acids 1 to 883 of SEQ ID NO:2.
An isolated polynucleotide comprising a DNA sequence or an RNA sequence obtained by screening an appropriate library, bacterial cell or bacterial cell lysate containing the complete gene encoding the amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a probe having a polynucleotide sequence encoding said amino acid sequence or a fragment thereof, which fragment retains binding or catalytic activity;
- and isolating said DNA sequence or RNA sequence.
An isolated polynucleotide comprising the polynucleotide sequence set forth in SEQ ID NO:1.
An isolated polynucleotide comprising a DNA sequence or an RNA sequence obtained by screening an appropriate library, bacterial cell or bacterial cell lysate containing the complete gene encoding the amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a probe having a polynucleotide sequence set forth in SEQ ID NO:1 or a fragment thereof, which fragment is a 17-mer or longer;
- and isolating said DNA sequence or RNA sequence.
An isolated polynucleotide of the formula:
- __________________________________________________________________________ X-(R.sub.1).sub.n -1 ATGTCTAAAG AACTTTCATC TAAATACAAT CCAGCCGAGG TTGAGGCTGG 51 TCGTTACCAA AAATGGCTTG ATGCTGATGT TTTCAAGCCT TCAGGCGATC 101 AAAAGGCTAA GCCTTATTCA ATCGTTATTC CACCACCAAA CGTTACAGGT 151 AAACTTCACC TTGGTCACGC TTGGGATACA ACTTTGCAAG ATATTATCAT 201 CCGTCAAAAA CGCATGCAAG GTTTTGATAC CCTTTGGCTT CCTGGGATGG 251 ACCACGCAGG GATTGCCACT CAGGCTAAGG TAGAGGAGCG CTTGCGTGGT 301 GAGGGCATTT CCCGCTATGA CCTTGGTCGT GAGTCTTTCT TGACGAAAGT 351 CTGGGAATGG AAAGACGAAT ATGCCACTAC TATCAAGGAA CAATGGGGCA 401 AGATGGGGCT CTCTGTAGAC TATTCTCGTG AGCGTTTCAC TCTTGACGAA 451 GGTTTGTCAA AAGCTGTTCG TAAGGTCTTT GTGGACCTTT ACAAGAAAGG 501 CTGGATCTAC CGTGGTGAGT TTATCATCAA CTGGGACCCA GCAGCTCGCA 551 CAGCCCTTTC TGATATTGAG GTGATTCACA AGGATGTGGA AGGTGCCTTC 601 TACCACATGA ATTACATGCT GGAAGATGGT TCACGCGTCC TTGAAGTTGC 651 TACAACTCGT CCTGAGACCA TGTTTGGGGA CGTTGCGGTT GCGGTCAACC 701 CAGAAGACCC GCGCTACAAG GACTTGATTG GTAAAAATGT CATCCTTCCA 751 ATCGCTAATA AACTCATCCC AATCGTTGGA GATGAGCACG CAGATCCTGA 801 GTTTGGTACT GGTGTCGTGA AAATCACACC TGCCCACGAT CCAAATGACT 851 TCTTGGTTGG CCAACGTCAT AACTTGCCAC AAGTCAACGT CATGAACGAC 901 GACGGAACCA TGAATGACTT GGCCTTTGAA TTTTCAGGCA TGGACCGTTT 951 TGAAGCTCGT AAGGCAGTCG TTGCTAAGTT GGAAGAAATC GGTGCCCTCG 1001 TCAAAATCGA AAAACGTGTC CACAGTGTTG GTCACTCAGA GCGTACAGGT 1051 GTTGTGGTTG AACCTCGCTT GTCTACTCAA TGGTTCGTCA AGATGGACCA 1101 ATTGGCTAAG AACGCCATTG CCAACCAAGA CACAGAGGAC AAGGTCGAAT 1151 TCTACCCACC TCGTTTCAAC GATACCTTCC TTCAATGGAT GGAAAATGTC 1201 CACGACTGGG TTATCTCTCG TCAGCTCTGG TGGGGTCACC AAATCCCTGC 1251 CTGGTACAAT GCTGATGGTG AAATGTATGT CGGCGAAGAA GCTCCAGAAG 1301 GTGACGGATG GACTCAGGAC GAAGACGTCT TGGATACTTG GTTCAGTTCT 1351 GCCCTCTGGC CATTTTCAAC CATGGGCTGG CCTGAAGTCG ACTCAGAAGA 1401 CTTTAAACGT TATTTCCCAA CTTCAACCTT GGTAACAGGT TACGACATCA 1451 TCTTCTTCTG GGTGTCTCGT ATGATCTTCC AGTCATTGGA ATTCACAGGC 1501 CGTCAACCAT TCCAAAACGT CCTTATCCAC GGTCTCATTC GTGACGAGCA 1551 AGGACGCAAG ATGTCTAAGT CTCTCGGTAA CGGGATTGAC CCAATGGATG 1601 TTATCGAGAA ATACGGTGCC GATGCCCTTC GATGGTTCCT TTCAAACGGT 1651 TCTGCGCCAG GACAAGACGT GCGTTTCTCT TATGAGAAAA TGGATGCTTC 1701 ATGGAACTTT ATTAACAAGA TTTGGAACAT TTCTCGCTAT ATCCTCATGA 1751 ACAATGGAGG TTTGACGCTG GATGTGGCGC ATGACAATGT CACAAAAGTT 1801 GCAACAGGTG AGGCTGGTAA TGTGACGGAC CGCTGGATTC TCCACAATCT 1851 CAACGAAACC ATTGCAAAAG TTACTGAAAA CTTTGATAAG TTCGAGTTTG 1901 GTGTGGCTGG TCATATCCTT TACAACTTCA TTTGGGAAGA ATTTGCCAAC 1951 TGGTATGTTG AGTTGACCAA GGAAGTTCTT TACAGCGACA ATGAAGACGA 2001 TAAGGTAATT ACTCGATCTG TTCTCCTCTA TACGCTGGAT AAAATCCTTC 2051 GTCTCCTTCA CTCAATAATG CCGTTCGTGA CAGAAGAAAT CTTTGGTCAG 2101 TACGCTGAAG GCTCTATCGT GACAGCAGCA TACCCAACTG TCAACCCAGC 2151 CTTTGAGGAC CTTGCTGCCC ACACTGGTGT GGAAAGTCTC AAAGACTTGA 2201 TCCGTGCCGT TCGTAATGCG CGTGCGGAAG TAAATGTAGC ACCAAGCAAA 2251 CCTATCACCA TCCTTGTTAA GACTAGCGAT AGTGACTTGG AAGCCTTCTT 2301 TAACAGCAAT GTCAACTACA TCAAACGCTT TACAAATCCA GAACACTTGG 2351 AAATCGCATC AACCATCCCT GCACCTGAAC TGGCTATGTC AAGCGTTATT 2401 ACAGGAGCCG AAATCTTCTT GCCACTGGTA GACCTCTTGA ATGTCGAAGA 2451 AGAATTGGCA CGTCTGGAAA AAGAACTTGC CAAATGGCAG AAAGAACTCG 2501 ACATGGTTGG CAAAAAACTC AGCAACGAAC GCTTCGTCGC CAACGCTAAA 2551 CCAGAAGTTG TCCAAAAAGA AAAAGACAAA CAAGCCGACT ACCAAGCCAA 2601 GTATGATGTG ACCGTAGCAC GTATTGATGA GATGAAGAAG TTGGTGAAAT 2651 AA-(R.sub.2).sub.n -Y __________________________________________________________________________
- wherein, at the 5' end of the molecule, X is hydrogen, and at the 3' end of the molecule, Y is hydrogen or a metal, R.sub.1 and R.sub.2 is any nucleic acid residue, and n is an integer between 1 and 1000, 2000 or 3000.
An isolated polynucleotide comprising a DNA sequence or an RNA sequence obtained by screening an appropriate library, bacterial cell or bacterial cell lysate containing the complete gene encoding an amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a primer or probe comprising the polynucleotide sequence set forth in SEQ ID NO:
- 3 or 4;
- and isolating said DNA sequence or RNA sequence.
An isolated polynucleotide comprising a polynucleotide encoding the same mature valyl tRNA synthetase polypeptide in deposit number NCIMB 40794 or NCIMB 40800.
An isolated polynucleotide comprising the polynucleotide sequence set forth in SEQ ID NO:3 or 4.
An isolated polynucleotide consisting of the polynucleotide sequence set forth in SEQ ID NO:3 or 4.
An isolated polynucleotide comprising a polynucleotide sequence encoding at least 30 contiguous amino acids of the sequence set forth in SEQ ID NO:2.
An isolated polynucleotide comprising a polynucleotide sequence encoding at least 50 contiguous amino acids of the sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.